Antibodies to Protein Phosphatase 2 family - FocusOn 039

Introduction: Protein phosphorylation is dependent on the opposing actions of protein kinases and protein phosphatases. Four major protein phosphatases have been identified in the cytosol of eukaryotic cells which are responsible for the dephosphorylation of serine and threonine residues in proteins. Although all 4 protein phosphatases, PP1, PP2A, PP2B, and PP2C, have overlapping substrate specificities in vitro, they can be distinguished by the use of inhibitor proteins and by their dependence on metal ions.

Protein Phosphatase 2A

Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. Dephosphorylation of substrates targeted by PP2A plays a vital role in a large variety of cellular processes including signal transduction, regulation of the cell cycle, gene expression and development. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.

AP19055PU-N PPP2CA antibody staining of Methanol-fixed HeLa at 1/200 dilution.
Fig.1: Cat.-No. AP19055PU-N PPP2CA antibody staining of Methanol-fixed HeLa at 1/200 dilution.
AP15290PU-N PPP2CA/PPP2CB antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue followed by peroxidase conjugated secondary antibody and DAB staining.
Fig.2: Cat.-No. AP15290PU-N PPP2CA/PPP2CB antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue followed by peroxidase conjugated secondary antibody and DAB staining.
AP05040PU-N PPP2R1A antibody staining of total Rat brain homogenate.
Fig.3: Cat.-No. AP05040PU-N PPP2R1A antibody staining of total Rat brain homogenate.
AP08886PU-N PPP2R3A antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, cerebellum at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Fig.4: Cat.-No. AP08886PU-N PPP2R3A antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, cerebellum at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Protein Phosphatase 2B

Calmodulin-dependent protein phosphatase, calcineurin or Protein Phosphatase 2B, is involved in a wide range of biologic activities, acting as a Ca(2+)-dependent modifier of phosphorylation status. In testis, the motility of the sperm is thought to be controlled by cAMP-dependent phosphorylation and a unique form of calcineurin appears to be associated with the flagellum. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. At least 3 genes, calcineurin A-alpha (PPP3CA), calcineurin A-beta (PPP3CB), and calcineurin A-gamma (CALNA3 or PPP3CC), have been cloned for the catalytic subunit. These genes have been identified in humans, mice, and rats, and are highly conserved between species (90 to 95% amino acid identity).

AP15297PU-N PPP3CC antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue, followed by peroxidase conjugated secondary antibody and AEC staining.
Fig.5: Cat.-No. AP15297PU-N PPP3CC antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue, followed by peroxidase conjugated secondary antibody and AEC staining.
AP09004PU-N PPP3R1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Fig.6: Cat.-No. AP09004PU-N PPP3R1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Protein Phosphatase 2C

PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.

Protein phosphatase 1B (PPM1B) is a member of the PP2C family of serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death.

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome.

PPM1M is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase was identified as an interacting protein of Rho guanine nucleotide exchange factors (PIX). PIX proteins are regulators of p21/Cdc42/Rac1-activated kinase 1 (PAK1), a protein kinase mediating biological effects downstream of Rho GTPases. This phosphatase has been shown to block the effects of PAK, and thus inhibit actin stress fiber breakdown and morphological changes driven by cell division cycle 42 (CDC42).

PPM1D is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. Expression of this PPM1D gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negativelyregulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stressinduced apoptosis. The PPM1D gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. 

Fig.7: Immunohistochemical staining of paraffin embedded human breast cancer sections using anti-PPM1G antibody Cat.-No. AM09028PU-N. Antibody dilution 1:50, 2 hours incubation time at RT. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Detection by Diaminobenzidine (DAB).
Fig.7: Immunohistochemical staining of paraffin embedded human breast cancer sections using anti-PPM1G antibody Cat.-No. AM09028PU-N. Antibody dilution 1:50, 2 hours incubation time at RT. Antigen retrieval was performed in 0.1 M sodium citrate buffer. Detection by Diaminobenzidine (DAB).
  

Available Antibodies to Protein Phosphatase 2 family

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (2)

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP2CA antibody

Immunohistochemistry analysis of PP2A-alpha antibody (Cat.-No AP06572PU-N) in Paraffin-embedded Human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PPP2CA antibody

Figure 1. Western blot analysis of extracts from various cells using PP2A-alpha antibody AP08081PU (Lane 1, 2, 3, 4 and 5). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PPP2CA antibody

Figure 1. Western blot analysis of extracts from various cells using PP2A-alpha antibody AP08081PU (Lane 1, 2, 3, 4 and 5). Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

PPP2R1B antibody

PP 2 A/B alpha western blot of total rat brain homogenate. (+) indicates presence of specific blocking peptide of control peptide (-). Rabbit IgG Purified Bov, Hu, Ms, Rt C, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP2R1B antibody

PP 2 A/B alpha western blot of total rat brain homogenate. Sheep IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP2R1B antibody

Figure 2. Staining PPP2R1B in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Bov, Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPP2R1B (C-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPP2R2B (110-139) antibody

AP18157PU-N PPP2R2B Antibody staining of Formalin-fixed and paraffin-embedded human brain tissue using peroxidase-conjugated secondary antibody, followed by DAB staining. Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPP2R2B antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1F3 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

PPP2R2B antibody

PP2 A/B western blot of total rat brain extract. (+) or (-) indicates present of blocking peptide or control peptide. Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP2R3A / PPP2R3 (1073-1085) antibody

Immunohistochemistry: AP08886PU-N PPP2R3A  antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, cerebellum at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat IgG Aff - Purified Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPP2R4 / PTPA antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining. Mouse IgG1 4D9 Purified Hu E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PPP2R5A (C-term) antibody

Western blot analysis of PPP2R5A Antibody (C-term) Cat.-No AP53420PU-N in mouse liver tissue lysates (35ug/lane). This demonstrates the PPP2R5A antibody detected the PPP2R5A protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPP2R5C antibody

Western blot analysis using anti-PP2A/Bg2 antibody on total rat brain homogenate with control peptide (1) and blocking peptide (2). Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP2R5D antibody

PPP2R5D Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

PPP2R5D (544-593) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PPP3CC / CALNA3 (C-term) antibody

Immunohistochemistry: AP15297PU-N PPP3CC antibody staining of Formalin-Fixed Paraffin-Embedded Human cancer tissue, followed by peroxidase conjugated secondary antibody and AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PPP3R1 / Calcineurin B antibody

Immunohistochemistry: AP09004PU-N PPP3R1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Bov, Dros, Hu, Ms, Rt, Ye IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Protein phosphatase 1A / PPM1A antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4E11 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Protein phosphatase 1A / PPM1A antibody

Immunoblot analysis Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2C-alpha (1/250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2C-alpha(~43kDa) Mouse IgG2b p6c7 Purified Hu, Ms E, IP, P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn