Antibodies to Sox Proteins - FocusOn 115

Acris Antibodies Panel to Sox Proteins

Acris Antibodies offers a large selection of antibodies for detection of Sox proteins, suitable for immunohistochemistry on formalin-fixed, paraffin-embedded (FFPE) tissue sections, immunocytochemistry and Western blotting.

Introduction1

Sox genes encode a family of transcription factors that bind to the minor groove in DNA, and belong to a super-family of genes characterized by a homologous sequence called the HMG (high mobility group) box. This HMG box is a DNA binding domain that is highly conserved throughout eukaryotic species. Homologues have been identified in insects, nematodes, amphibians, reptiles, birds and a range of mammals. However, HMG boxes can be very diverse in nature, with only a few amino acids being conserved between species.

Sox genes are defined as containing the HMG box of a gene involved in sex determination called Sry, which resides on the Y-chromosome (Sox stands for Sry-related HMG box). There are 20 Sox genes present in humans and mice, and 8 present in Drosophila. Almost all Sox genes show at least 50 % amino acid similarity with the HMG box in Sry. The family is divided into subgroups according to homology within the HMG domain and other structural motifs, as well as according to functional assays [Bowles, 2000].

The developmentally important Sox family has no singular function, and many members possess the ability to regulate several different aspects of development. While many Sox genes are involved in sex determination, some are also important in processes such as neuronal development. For example, Sox2 and Sox3 are involved in the transition of epithelial granule cells in the cerebellum to their migratory state. Granule cells then differentiate to granule neurons, with Sox11 being involved in this process [Rex, 1998]. It is thought that some Sox genes may be useful in the early diagnosis of childhood brain tumors due to this sequential expression in the cerebellum, making them a target for significant research.

Sox  proteins bind to the sequence WWCAAW and similar sequences (W=A/T). They have weak binding specificity and unusual low affinity for DNA. Sox genes are related to the Tcf/Lef1 group of genes which also contain a sequence-specific high mobility group and have a similar sequence specificity (roughly TWWCAAAG) [Lefebvre, 2007].

1 http://en.wikipedia.org/wiki/SOX_genes (17. April 2011)

Fig. 1: Western blot analysis of Sox1 using Cat.-No. AP22237PU-N at 0.5 µg/ml, 0.5 µg/ml and 2 µg/ml respectively: Human brain lysate in the absence (1) and presence (2) of immunizing peptide and mouse brain lysate (3)
Fig. 1: Western blot analysis of Sox1 using Cat.-No. AP22237PU-N at 0.5 µg/ml, 0.5 µg/ml and 2 µg/ml respectively: Human brain lysate in the absence (1) and presence (2) of immunizing peptide and mouse brain lysate (3)
Fig. 2: Formalin-fixed and paraffin-embedded (FFPE) human lung carcinoma tissue reacted with Sox2 monoclonal antibody Cat.-No. AM11050PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 2: Formalin-fixed and paraffin-embedded (FFPE) human lung carcinoma tissue reacted with Sox2 monoclonal antibody Cat.-No. AM11050PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 3: Immunofluorescence analysis in HeLa cells using Sox2 monoclonal antibody Cat.-No. AM11050PU-N (25 µg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (orange; blue counterstaining with DAPI)
Fig. 3: Immunofluorescence analysis in HeLa cells using Sox2 monoclonal antibody Cat.-No. AM11050PU-N (25 µg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (orange; blue counterstaining with DAPI)
Fig. 4: Immunohistochemical analysis of Sox9 in mouse testis tissue (FFPE) using an isotype control (top left) and Sox9 antibody Cat.-No. AP22243PU-N (bottom left, right) at 5 µg/ml
Fig. 4: Immunohistochemical analysis of Sox9 in mouse testis tissue (FFPE) using an isotype control (top left) and Sox9 antibody Cat.-No. AP22243PU-N (bottom left, right) at 5 µg/ml
Fig. 5: Immunoperoxidase staining of monoclonal Sox10 antibody Cat.-No. H00006663-M01 on human colon (FFPE; antibody concentration 1.5 µg/ml)
Fig. 5: Immunoperoxidase staining of monoclonal Sox10 antibody Cat.-No. H00006663-M01 on human colon (FFPE; antibody concentration 1.5 µg/ml)
Fig. 6: Human fetal brain (FFPE) stained with Sox14 antibody Cat.-No. AP15786PU-N
Fig. 6: Human fetal brain (FFPE) stained with Sox14 antibody Cat.-No. AP15786PU-N
Fig. 7: Immunofluorescence on MCF-7 cell using monoclonal antibody to Sox30 Cat.-No. H00011063-M02 (antibody concentration 10 µg/ml)
Fig. 7: Immunofluorescence on MCF-7 cell using monoclonal antibody to Sox30 Cat.-No. H00011063-M02 (antibody concentration 10 µg/ml)

Key References

Bowles J et al: Phylogeny of the Sox family of developmental transcription factors based on sequence and structural indicators.
Dev Biol, Nov 2000; 227 (2): 239-55.
Rex M et al: Granule cell development in the cerebellum is punctuated by changes in Sox gene expression.
Molecular Brain Research, Mar 1998; 55 (1): 28-34.
Lefebvre V et al: Control of cell fate and differentiation by Sry-related high-mobility-group box (Sox) transcription factors.
Int J Biochem Cell Biol, 2007; 39 (12): 2195-214.
Guth SI et al: Having it both ways: Sox protein function between conservation and innovation.
Cell Mol Life Sci, Oct 2008; 65 (19): 3000-18.

Further readings

See the PDF-version: FocusOn 115

  1. Quick-Filters:
  2. Cited (1)
  3. Overnight Delivery (1)

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOX2 (N-term) antibody

AM09112PU SOX2 antibody staining of Human newborn brain. Rabbit IgG SP76 Purified Hu P, WB
1 ml / €730.00
  Acris Antibodies GmbH

SOX2 (N-term) antibody

AM09112PU SOX2 antibody staining of Human newborn brain. Rabbit IgG SP76 Purified Hu P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

SOX2 (N-term) antibody

Human skin stained with anti-SOX2 antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SOX2 (N-term) antibody

Human skin stained with anti-SOX2 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SOX2 (N-term) antibody

Figure 1. AP08354PU-N SOX2 antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, Cortex at 1/50 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SOX3 antibody

SOX3 monoclonal antibody (M04), clone 1F2 Western Blot analysis of SOX3 expression in A-431 ( Cat # L015V1 ). Mouse IgG2a 1F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SOX6 antibody

Western blot (WB) analysis of Sox-6 antibody (Cat.-No.: AP20382PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SOX8 antibody

Western Blot analysis of SOX8 expression in transfected 293T cell line (H00030812-T01) by SOX8 MaxPab polyclonal antibody.

Lane 1: SOX8 transfected lysate(49.06 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SOX9 antibody

Immunohistochemistry analysis of SOX9 Antibody (Cat.-No AP06583PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SOX9 antibody

Human testis stained with Anti-SOX9 antibody (Cat#AP15790PU). Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SOX9 antibody

Human testis stained with Anti-SOX9 antibody (Cat#AP15790PU). Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SOX9 antibody

AP15791PU SOX9 antibody staining of Human Testis. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SOX9 antibody

AP15791PU SOX9 antibody staining of Human Testis. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SOX9 antibody

Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7 ug/ml] Mouse IgG2a 3C10 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SOX9 antibody

Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5 ug/ml] Mouse IgG1 3F11 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SOX10 antibody

Immunoperoxidase of monoclonal antibody to SOX10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml] Mouse IgG2a 1E6 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SOX13 antibody

Immunofluorescence of purified MaxPab antibody to SOX13 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

SOX14 antibody

AP15786PU-N SOX14 antibody staining of Human brain. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SOX14 antibody

AP15786PU-N SOX14 antibody staining of Human brain. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SOX15 antibody

Immunoperoxidase of monoclonal antibody to SOX15 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 3 ug/ml] Mouse IgG2a 2C1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn