Antibody Panel to ink4 Protein Family - FocusOn 061

Introduction

Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. The D type cyclins complex with CDK4 and CDK6 to govern progression through the G1 phase of the cell cycle and later are involved with inactivating phosphorylation of the RB protein, which results in release of RB-associated transcription factors that are needed for entry into S phase. The activity of the cyclin D-dependent kinases is, in part, controlled by inhibitors such as the INK4 family.

INK4 family includes INK4a (CDKN2A); 4b (CDKN2B); 4c (CDKN2C), and 4d (CDKN2D).

Reportedly, p15INK4b expression is induced approximately 30-fold in human keratinocytes by treatment with TGF-beta, suggesting that p15INK4b may act as an effector of TGF-Beta-mediated cell cycle arrest. p18INK4c and p19INK4d are expressed during G1 -to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.



Antibody Tools for the Detection of ink4

Acris Antibodies offers a panel of antibodies to ink4 proteins which are suitable for Western blotting, immunohistochemistry or other detection methods.



Formalin-fixed, paraffin-embedded human colon carcinoma stained with p15INK4b antibody (DM379) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Fig. 1

Formalin-fixed, paraffin-embedded human tonsil stained with p18INK4c (AM12104PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

Fig. 2

Fig. 1: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p15INK4b antibody (DM379) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Fig. 2: Formalin-fixed, paraffin-embedded human tonsil stained with p18INK4c (AM12104PU-N) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.



Available Antibody Panel to ink4 Protein Family

Anti- p15INK4b (CDKN2B, MTS2), p18INK4C (CDKN2C, CDKN6), p19INK4d (CDKN2D) antibody

5 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDKN2B / p15INK4b antibody

CDKN2B / p15INK4b Mouse IgG1 DCS-114.1 Purified Hu C, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

CDKN2C / p18INK4c antibody

Figure 3. AM00301AF-N p18INK4c antibody Staining of Paraffin Embedded Human Tonsil Section (germinal center). Mouse IgG2a DCS-118 Azide Free Hu IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN2C / p18INK4c antibody

Figure 1. AM12104PU-N p18INK4c antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells. Mouse IgG2a DCS118 Purified Hu P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CDKN2D / p19INK4d antibody

Figure 1. AM05227AF-N p18INK4d antibody staining of Paraffin Embedded of Human Tonsil Section (germinal center). Mouse IgG1 DCS-100 Azide Free Hu IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CDKN2D / p19INK4d antibody

CDKN2D / p19INK4d Mouse IgG1 DCS-100 Purified Hu C, IP, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

5 Item(s)

per page
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