Antibody Panel to Insulin Receptor (CD220) - FocusOn 060

Introduction

The insulin receptor is a transmembrane protein which consists of 4 subunits (2 alpha 2 beta) and exhibits receptor tyrosine kinase activity (RTK).

RTKs are single-pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma-phosphate of ATP to tyrosine residues in protein substrates.

RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism.

Included in this large protein family are the insulin receptor (IR, INSR) and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.

Antibody Tools for the Detection of CD220

Acris Antibodies offers a panel of antibodies for the detection of Insulin Receptor (alpha / beta).

These antibodies can be used for immunohistochemistry, Western blotting or other immunological methods.

Formalin-fixed, paraffin-embedded human placenta stained with anti-Insulin Receptor antibody AM00362PU-N using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts.Paraffin embedded pancreas: Islet of Langerhans stained with anti-Insulin Receptor antibody AP00470PU-N

Fig.1: Formalin-fixed, paraffin-embedded human placenta stained with anti-Insulin Receptor antibody AM00362PU-N using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts.

Fig.2: Paraffin embedded pancreas: Islet of Langerhans stained with anti-Insulin Receptor antibody AP00470PU-N

Available Antibody Panel to Insulin Receptor

Insulin Receptor (CD220, IR, INSR) antibody, Insulin Receptor alpha and Insulin Receptor beta antibody

10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD220 / INSR pTyr1150/1151 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of Control (Lane 1) or Insulin stimulated (Lane 2) MDA-MB-213 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. 
The Immunoblot was probed with Monoclonal InsR antibody 10C3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 10C3 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1322 (incl. pos. control) antibody

Phosphospecificity: Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: untreated 
Lane 2: Insulin-treated 
Lane 3: IGF1-treated
Lane 4: Pervanadate-treated Mouse IgG1 21G12 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). 
Lane 1: A431 
Lane 2: SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF-7 
Lane 6: MDA-MB231 
Lane 7: T47D Mouse IgG1 9H4 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-11B6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A Mouse IgG1 11B6 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1185 antibody

Dot blot analysis of anti-Phospho-INSR pTyr1185 Antibody (Cat.#AP12966PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

CD220 / INSR (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD220 / INSR (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with INSR polyclonal antibody ( Cat # PAB3356 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

CD220 / INSR (beta chain) antibody

Western Blot: Insulin Receptor beta Antibody [NB120-10991] - Representative lot data. Human brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Insulin Receptor, b subunit (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.  Arrow indicates Insulin Receptor, b subunit (~97kDa) Rabbit IgG Aff - Purified Hu E, P, WB
50 µl / €390.00
  Novus Biologicals Inc.

CD220 / INSR (alpha chain) antibody

CD220 / INSR Mouse IgG2b MA-20 Purified Hu, Rt E, IP, NEUT, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

CD220 / INSR (alpha chain) antibody

CD220 / INSR Mouse IgG2b Aff - Purified Hu, Rt E, IP, NEUT, WB
25 µl / €200.00
  Novus Biologicals Inc.

10 Item(s)

per page
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