Antibody Panel to Members of the Forkhead Box (FOX) Transscription Factor Family - FocusOn 124

Introduction

FOX transcription factors are characterized by the FOX domain, which is an ~100-amino-acid monomeric DNA binding domain. The three-dimensional structure of FOX domain consists of two W1 and W2 loops (or wings) and three helices. Due to the butterfly-like appearance, FOX domain is also known as ‘winged-helix’ domain.

Forkhead-box (FOX) gene family consists of at least 43 members, including FOXA1, FOXA2, FOXA3, FOXB1, FOXC1, FOXC2, FOXD1, FOXD2, FOXD3, FOXD4, FOXD5 (FOXD4L1), FOXD6 (FOXD4L3), FOXE1, FOXE2, FOXE3, FOXF1, FOXF2, FOXG1 (FOXG1B), FOXH1, FOXI1, FOXJ1, FOXJ2, FOXJ3, FOXK1, FOXK2, FOXL1, FOXL2, FOXM1, FOXN1, FOXN2 (HTLF), FOXN3 (CHES1), FOXN4, FOXN5 (FOXR1), FOXN6 (FOXR2), FOXO1 (FOXO1A), FOXO2 (FOXO6), FOXO3 (FOXO3A), FOXO4 (MLLT7), FOXP1, FOXP2, FOXP3, FOXP4, and FOXQ1. FOXE3-FOXD2 (1p33), FOXQ1-FOXF2-FOXC1 (6p25.3), and FOXF1-FOXC2-FOXL1 (16q24.1) loci are FOX gene clusters within the human genome. Members of FOX subfamilies A-G, I-L and Q were grouped into class 1 FOX proteins, while members of FOX subfamilies H and M-P were grouped into class 2 FOX proteins. C-terminal basic region within the FOX domain was the common feature of class 1 FOX proteins. FOXH1 and FOXO1 mRNAs are expressed in human embryonic stem (ES) cells. FOXC1, FOXC2, FOXE1, FOXE3, FOXL2, FOXN1, FOXP2 and FOXP3 genes are mutated in human congenital disorders. FOXA1 gene is amplified and over-expressed in esophageal and lung cancer. FOXM1 gene is up-regulated in pancreatic cancer and basal cell carcinoma due to the transcriptional regulation by Sonic Hedgehog (SHH) pathway. FOXO1 gene is fused to PAX3 or PAX7 genes in rhabdomyosarcoma. FOXO3 and FOXO4 genes are fused to MLL gene in hematological malignancies. Deregulation of FOX family genes leads to congenital disorders, diabetes mellitus, or carcinogenesis.

The forkhead (Fox) gene family comprises a diverse group of winged-helix transcription factors that play important roles in development, metabolism, cancer and aging. Recently, several forkhead genes have been demonstrated to play critical roles in lymphocyte development and effector function, including FoxP3 in the development of regulatory T cells, FoxJ1 and FoxO3a in the regulation of CD4+ T cell tolerance, and FoxN1 in thymic development. Roles for other forkhead genes have also been proposed, including FoxP1 in macrophage differentiation, FoxP1 in natural killer cell effector function and FoxD2 in T cell activation. Thus, forkhead genes promise insight into the mechanisms of immunoregulation in several immune cell lineages, and their dysregulation likely contributes to the pathogenesis of several immunological disorders, suggesting that their study will lead to the development of novel therapeutic agents.

Antibody Tools for Detection of Forkhead box Proteins

Acris Antibodies offers a wide range of mono- and polyclonal antibodies for the detection of members of the FOX transcription factor family. The antibodies are suitable for Western blotting, some of them can be used for immunofluorescence and / or immunohistochemical stainings of Paraffin embedded tissues.



Figure 1 shows immunohistochemical staining of FOXL2 in murine prenatal follicle using antibody SP5291P

Figure 2 shows immunofluorescence of FOXL2 in KK1 granulosa cells using antibody SP5291P

Fig.1: shows immunohistochemical staining of FOXL2 in murine prenatal follicle using antibody SP5291P

Fig.2: shows immunofluorescence of FOXL2 in KK1 granulosa cells using antibody SP5291P

Staining of human lung (alveolar cells) using anti-FOXA3 antibody ARP31945: positive label indicated wit arrows

Staining of human hepatocytes using anti-FOXG1A (Forkhead-related protein FKHL2) antibody P100984: positive label indicated with arrows

Fig.3: Staining of human lung (alveolar cells) using anti-FOXA3 antibody ARP31945: positive label indicated wit arrows

Fig.4: Staining of human hepatocytes using anti-FOXG1A (Forkhead-related protein FKHL2) antibody P100984: positive label indicated with arrows

Staining of human kidney (epithelial cells of renal tubule) using anti-FOXF2 antibody ARP31694: positive label indicated with arrows

Staining of human stomach (fundic gland) using anti-FOXJ2 antibody ARP32910: positive label indicated wit arrows

Fig.5: Staining of human kidney (epithelial cells of renal tubule) using anti-FOXF2 antibody ARP31694: positive label indicated with arrows

Fig.6: Staining of human stomach (fundic gland) using anti-FOXJ2 antibody ARP32910: positive label indicated wit arrows



Available Antibodies to FOX Transscription Factor Family

Anti- FOXA1, FOXA2, FOXA3, FOXB1, FOXC1, FOXC2, FOXD1, FOXD2, FOXD3, FOXE1 antibody, FOXE2, FOXE3, FOXF1, FOXF2, FOXG1 (FOXG1B), FOXH1, FOXI1, FOXJ2, FOXK2, FOXL1, FOXL2, FOXM1, FOXN1 antibody, FOXN2 (HTLF), FOXN3 (CHES1), FOXO1 (FOXO1A), FOXO2 (FOXO6), FOXO3 (FOXO3A), FOXP1, FOXP2 antibody, FOXP3, FOXP4, FOXQ1 antibody and FOXR2 (FOXN6) antibody



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FOXA1 / TCF3A (C-term) antibody

AP09584PU FOXA1 antibody staining of Human Breast Carcinoma. Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

FOXA1 / TCF3A (C-term) antibody

AP09584PU FOXA1 antibody staining of Human Breast Carcinoma. Rabbit IgG Aff - Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

FOXA1 / TCF3A antibody

MCF7; WB Suggested Anti-FOXA1 Antibody . . Positive Control: Lane 1: 20ug MCF7 cell lysates Lane 2: 20ug MCF7 cell lysates Lane 3: 20ug MCF7 cell lysates Lane 4: 20ug MCF7 cell lysates Lane 5: 20ug MCF7 with FoxA1 knockdown Lane 6: 20ug MCF7 with FoxA1 knockdown . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:2000. Submitted by: Fu, Xiaoyong, Baylor College of Medicine. FOXA1 is supported by BioGPS gene expression data to be expressed in MCF7.; FOXA1 antibody - C-terminal region (ARP32630_P050) in MCF7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

FOXA1 / TCF3A antibody

ELISA: foxa1 Antibody [21560002] Rabbit Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

FOXA1 / TCF3A antibody

Immunohistochemistry-Paraffin: FOXA1 Antibody [NB100-1262] - (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu, Ms ChIP, E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FOXA1 / TCF3A antibody

Western Blot: FOXA1 Antibody [NBP1-30877] - Whole cell lysate treated with FOXA1 Antibody, diluted at 1:1000 with 10% SDS PAGE gel. Rabbit IgG Hu Dot, E, ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

FOXA2 / HNF3B antibody

Human Jurkat; WB Suggested Anti-FOXA2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate; FOXA2 antibody - N-terminal region (ARP39508_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXA2 / HNF3B antibody

Human HepG2; WB Suggested Anti-FOXA2 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. FOXA2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; FOXA2 antibody - C-terminal region (ARP39509_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXA2 / HNF3B antibody

HNF-3? in human HepG2 cells.  Cells were stained using Rhodamine Red-conjugated donkey anti-goat secondary antibody . Goat IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €450.00
  Neuromics Antibodies

FOXA2 / HNF3B antibody

Western Blot: FOXA2 Antibody [NB100-1263] - NB100-1263 staining (1µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FOXA3 / HNF3G antibody

Human Lung; FOXA3 antibody - middle region (ARP31945_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

FOXB1 / FKH5 antibody

Human HepG2; WB Suggested Anti-FOXB1 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; FOXB1 antibody - N-terminal region (ARP38961_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Dros, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXB1 / FKH5 antibody

FOXB1 / FKH5 Goat IgG Aff - Purified Ms E
0.1 mg / €400.00
  Novus Biologicals Inc.

FOXC1 / FKHL7 / FREAC3 (250-300) antibody

Colon: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Serum Bov, Hu, Mky, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FOXC1 / FKHL7 / FREAC3 antibody

Human HepG2; WB Suggested Anti-FOXC1 Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; FOXC1 antibody - C-terminal region (ARP32300_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXC1 / FKHL7 / FREAC3 antibody

Immunohistochemistry: FOXC1 Antibody [NB100-1268] -  FOXC1 (C Term) Antibody [NB100-1268]-(3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining Goat IgG Aff - Purified Hu, Ms, Ze ChIP, E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FOXC1 / FKHL7 / FREAC3 antibody

Immunohistochemistry: FoxC1/2 Antibody [NBP1-19590] -  FoxC1/2 Antibody [NBP1-19590] - paraffin-embedded human prostate carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

FOXC2 / FKHL14 / MFH1 antibody

Human Jurkat; WB Suggested Anti-FOXC2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; FOXC2 antibody - C-terminal region (ARP31703_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXC2 / FKHL14 / MFH1 antibody

Human kidney; WB Suggested Anti-FOXC2 Antibody Titration: 1.25ug/ml. Positive Control: Human Kidney; FOXC2 antibody - middle region (ARP32307_T100) in Human kidney cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

FOXC2 / FKHL14 / MFH1 antibody

Western Blot: FOX C2 Antibody [NB100-1269] - NB100-1269 staining (1ug/ml) of Human Breast Cancer lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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