Antibody Panel to Thyroid Hormone Receptor (TR) - FocusOn 062

Introduction

Thyroid hormone receptors (TR) are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone.

Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.

Antibody Tools for the Detection of Thyroid Hormone Receptor Subtypes

A panel of monoclonal and polyclonal antibodies for detection of Thyroid Hormone Receptor is available at Acris Antibodies. These antibodies are suitable for several immunological applications including immunohistochemistry on paraffin embedded sections.

Formalin fixed paraffin embedded human colon carcinoma stained with THR a1/a2 antibody DP149 using ABC and AEC chromogen.

Fig. 1: Formalin fixed paraffin embedded human colon carcinoma stained with THR a1/a2 antibody DP149 using ABC and AEC chromogen.

Available Antibody Panel to Thyroid Hormone Receptor

Anti- Thyroid Hormone Receptor (TR), TR alpha (THRA), TR alpha 1, TR alpha 2, TR beta (THRB) antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

THRA / ERBA1 (alpha-1 + alpha-2) antibody

AP15818PU THR alpha1/2 antibody staining of Human Thyroid. Rabbit Purified Hu, Ms, Por, Rt P
1 ml / €440.00
  Acris Antibodies GmbH

THRA / ERBA1 (alpha-1 + alpha-2) antibody

AP15818PU THR alpha1/2 antibody staining of Human Thyroid. Rabbit Purified Hu, Ms, Por, Rt P
0.1 ml / €180.00
  Acris Antibodies GmbH

THRA / ERBA1 (alpha-1 + alpha-2) antibody

AP15818PU THR alpha1/2 antibody staining of Human Thyroid. Rabbit Purified Hu, Ms, Por, Rt P

Regular Price: 50 µl / €80.00

Special Price: 50 µl / €56.00

  Acris Antibodies GmbH

THRA / ERBA1 (alpha-2) antibody

THRA / ERBA1 Mouse IgG1 1330 Purified Bov, Can, Hu, Ms, Rt WB
0.1 ml / €490.00
  Acris Antibodies GmbH

THRA / ERBA1 antibody

Immunohistochemistry analysis of THRA / ERBA1 Antibody (Cat.-No AP06353PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

THRA / ERBA1 antibody

WB using Anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

THRA / ERBA1 antibody

Western Blot analysis of THRA expression in transfected 293T cell line (H00007067-T02) by THRA MaxPab polyclonal antibody.

Lane 1: THRA transfected lysate(46.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

Western blot of hippocampal lysate showing specific immunolabeling of the ~58k THRA protein. Using THRA monoclonal antibody, clone 1330 ( Cat # MAB1781 ). Mouse IgG1 1330 Bov, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

Western blot of hippocampal lysate showing specific immunolabeling of the ~50k THRA and the ~58k THRA isotype. Using THRA monoclonal antibody, clone 1718 ( Cat # MAB1782 ). Mouse IgG2a 1718 Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

Western blot of hippocampal lysate showing specific immunolabeling of the ~50k THRA and the ~58k THRA isotype. Using THRA monoclonal antibody, clone 2103 ( Cat # MAB1783 ). Mouse IgG 2103 Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

THRA / ERBA1 (alpha-1 + alpha-2) antibody

THRA polyclonal antibody ( Cat # PAB7338 ) ( 0.5 µg/ml ) staining of human bone marrow lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

Western blot using THRA polyclonal antibody ( Cat # PAB11276 ) shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA ( lane 3 ).
No staining is evident in lysates from mock-transfected 293 cells ( lane 2 ).
Endogenous THRA is not detected in mouse brain tissue lysate ( lane 4 ).
Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus.
The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD. Rabbit Hu, Ms, Rt E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

THRA / ERBA1 Rabbit Hu P
0.1 ml / €390.00
  Abnova Taiwan Corp.

THRA / ERBA1 antibody

Human Jurkat; WB Suggested Anti-THRA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that THRA is expressed in Jurkat.; THRA antibody - middle region (ARP34780_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

THRA / ERBA1 (alpha-1 + alpha-2) antibody

Formalin fixed paraffin embedded human Colon carcinoma stained with THR a1/a2 using ABC and AEC chromogen. Rabbit Aff - Purified Hu, Por, Rt P
0.5 ml / €400.00
  Novus Biologicals Inc.

THRA / ERBA1 (alpha-2) antibody

Western Blot: Thyroid Hormone Receptor alpha Antibody (1330) [NB100-61087] - Hippocampal lysate showing specific immunolabeling of the ~58k TR-á2 protein. Mouse IgG1 1330 Purified Hu WB
0.1 ml / €380.00
  Novus Biologicals Inc.

THRA / ERBA1 (alpha-1 + alpha-2) antibody

NB100-68156 (0.5µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

THRA / ERBA1 antibody

Immunohistochemistry-Paraffin: Thyroid Hormone Receptor alpha Antibody [NB100-91998] - Paraffin-embedded human colon carcinoma tissue. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

THRA / ERBA1 antibody

Western Blot: Thyroid Hormone Receptor alpha Antibody [NB110-93504] - Hippocampal lysate showing specific immunolabeling of the ~50k TR-a1 and the ~58k TR-a2 protein. Mouse Aff - Purified Hu WB
0.1 ml / €380.00
  Novus Biologicals Inc.

THRA / ERBA1 (alpha-1) antibody

THRA / ERBA1 Rabbit IgG Serum Chk, Hu EMSA, ICC/IF, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

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