Antibody Panel to Tubulin Isoforms - FocusOn 067

Introduction

Microtubules constitute one of the major components of eukaryotic cell cytoskeleton and are involved in many essential processes, including cell division, ciliary and flagellar motility and intracellular transport. Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of alpha- and beta-tubulin. In addition to alpha- and beta-tubulin, several other tubulins have been identified.

Alpha and beta-tubulins alternate with one another in 13 linear filaments to form microtubules. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. The function of microtubules is dependent on their ability to grow and shrink. This dynamic instability is dependent on many factors, the main one being the concentration of GTP-bound tubulin heterodimers. Also, molecules called microtubule-associated proteins (MAPs) can inhibit or stimulate the polymerization of microtubules.

Class III beta tubulin is a vertebrate tubulin isotype only found in neurons and cells in the mammalian testis. Therefore, Class III beta tubulin antibodies are commonly used as neuronal markers.

Gamma-tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly.

Delta-tubulin function is an essential component during spermatogenesis.

Epsilon-Tubulin was found to be located in the centriolar area, a localization that is dependent on cell cycle modulation.

Tubulins are generally highly conserved proteins throughout all eukaryote species. Therefore, most available antibodies to tubulins are reactive on nearly all species tested so far.

Antibody Tools for the Detection of Tubulins

Acris Antibodies offers a wide range of anti-Tubulin antibodies, which are suitable for immunohistochemistry and / or other immunological detection methods.

Staining results for anti alpha-Tubulin antibody AM00286PU-N on human (left) and rat lung (paraffin embedded sections). Note cytplasmic staining of ciliated epithelium of bronchioles.Staining results for anti alpha-Tubulin antibody AM00286PU-N on human (left) and rat lung (paraffin embedded sections). Note cytplasmic staining of ciliated epithelium of bronchioles.Confocal image of in vitro rat hippocampal neurons using alpa Tubulin antibody YOL1/34 code SM568P (green, blue: MAP2) Staining of human lung using Beta Tubulin antibody BM2233 (paraffin embedded section)

Fig. 1/2: Staining results for anti alpha-Tubulin antibody AM00286PU-N on human (left) and rat lung (paraffin embedded sections). Note cytplasmic staining of ciliated epithelium of bronchioles.

Fig. 3: Staining of human lung using beta-Tubulin antibody BM2233 (paraffin embedded section).

Fig. 4: Staining of human neuronal progenitor cells using Class III beta-Tubulin antibody MO15013.

Available Antibody Panel to Tubulin

Anti- Tubulin, Tubulin alpha (TUBA1, TUBA1B, TUBA3), Tubulin beta (TUBB2, TUBB2A, TUBB2B, TUBB3 ), Tubulin delta (TUBD), Tubulin gamma (TUBG, TUBG2), Tubulin epsilon (TUBE) antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

alpha + beta Tubulin (heterodimer) antibody

alpha + beta Tubulin Mouse IgM TU-08 Ig Fraction Hu, Ms, Por ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha + beta Tubulin (heterodimer) antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb TU-10 and goat anti-mouse IgG (H+L)-HRP. Mouse IgM TU-10 Purified All Species ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1A / TUBA3 antibody

Immunohistochemistry: Tubulin alpha Antibody [NB100-92249] - Immunohistochemistry (IHC) analyzes of Tubulin á  (G436) antibody in paraffin-embedded human breast  carcinoma tissue. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA1B (Loading Control) antibody

Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green) and anti-Vimentin antibody (VI-01; SM3128P; red). Nucleus is stained with DAPI (blue). Mouse IgG1 TU-01 Purified All Species C, E, ICC/IF, IP, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Immunohistochemistry: SM568P Alpha Tubulin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rat IgG2a YOL1/34 Purified Av, Hu, Mam, Ms, Rt, Ye C, E, ICC/IF, IP, P, R, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Immunofluorescent: Tubulin Antibody (TU-01) [NB500-333] - Fig. 2. Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue). Mouse IgG1 TU-01 Aff - Purified Hu, Mam, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA1B (426-450) antibody

Human lung stained with Anti-Tubulin alpha antibody. Rabbit Purified Bov, Chk, Frog, Ge, GP, Hu, Ms, Por, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (426-450) antibody

Human lung stained with Anti-Tubulin alpha antibody. Rabbit Purified Bov, Chk, Frog, Ge, GP, Hu, Ms, Por, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (N-term) antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by  mAb TU-02 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgM TU-02 Purified Hu, Ms, Por WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

AM08323PU-N at 10 µg/ml staining alpha Tubulin in Pig heart by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue. Mouse IgM TU-16 Purified All Species E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Immunohistochemistry (IHC) analyzes of Tubulin alpha antibody (Cat.-No.: AP06604PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (427-441) (Loading Control) antibody

Figure 1. Staining Tubulin alpha in  Brain, cortex by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (Loading Control) antibody

alpha Tubulin / TUBA1B Mouse IgG1 TU-01 Biotin All Species WB
0.1 mg / €310.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (Loading Control) antibody

Immunofluorescence staining of 3T3 Mouse embryonal fibroblast cell line using anti-alpha-tubulin antibody (TU-01; green). Nucleus is stained with DAPI (blue). Mouse IgG1 TU-01 FITC All Species ICC/IF, P
0.1 mg / €290.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B (Tyr-Tubulin) antibody

HeLa whole cell lysate probed with Rat anti Tubulin Alpha antibody HRP conjugated. Rat IgG2a YL1/2 Purified Av, Mam, Ye C, E, ICC/IF, IP, R, WB
0.5 mg / €550.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions) Mouse IgM TU-16 Purified All Species C, E, ICC/IF, IP, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

alpha Tubulin / TUBA1B antibody

alpha Tubulin / TUBA1B Rat IgG2a YOL1/34 Purified Av, Dros, Fu, Hu, Mam, Ms, Rt, Ye C, E, ICC/IF, P, R, WB
0.25 mg / €360.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA1B antibody

alpha Tubulin / TUBA1B Mouse IgG1 B-5-1-2 Aff - Purified Bov, Chk, Hu, Mky, Ms, Rt ICC/IF, IP, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA1B antibody

Immunofluorescence: Tubulin alpha-1B chain Antibody [NBP1-32959] - Immunofluorescence analysis of  Tubulin alpha-1B chain on A431, using NBP1-32959. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

alpha Tubulin / TUBA3C / TUBA2 antibody

Human Hela; Host: Rabbit . Target Name: TUBA3C . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. TUBA3C is supported by BioGPS gene expression data to be expressed in HeLa.; TUBA3C antibody - N-terminal region (ARP53650_P050) in Human Hela cells using Western Blot Rabbit Aff - Purified Ce, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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