CD163: Marker for intermediate and late stage Inflammatory Macrophages - FocusOn 010

Introduction

CD163 is a highly expressed macrophage membrane protein belonging to the scavenger receptor cysteine rich (SRCR) domain family. The CD163 expression is induced by interleukin-6, interleukin-10 and glucocorticoids. Its function has remained unknown until recently when CD163 was identified as the endocytic receptor binding hemoglobin (Hb) in complex with the plasma protein haptoglobin (Hp). This specific receptor-ligand interaction leading to removal from plasma of the Hp-Hb complex-but not free Hp or Hb now explains the depletion of circulating Hp in individuals with increased intravascular hemolysis. The novel functional linkage of CD163 and Hp, which both are induced during inflammation, also reveal some interesting perspectives relating to the suggested anti-inflammatory properties of the receptor and the Hp phenotypes. Cross-linking of CD163 on glucocorticoid-stimulated macrophages results in the secretion of several proinflammatory cytokines, but the precise mechanism of CD163 mediated signal transduction is not understood. Recent studies showed that the regulatory beta-subunit of casein kinase II (CKII) specifically binds to the cytoplasmic domain of CD163. Furthermore, it was demonstrated that the cytoplasmic domains of CD163 variants are phosphorylated by PKC-alpha in vitro. Inhibition studies using specific kinase inhibitors revealed that both CKII and PKC are involved in the CD163 signaling mechanism resulting in the secretion of proinflammatory cytokines.

Antibody Tools for Detection of human CD163

Ki-M8 and RM3/1 were the first clones specific to human CD163. All the initial research on that protein was performed using these clones. Meanwhile Acris Antibodies offers a panel of monoclonal antibodies to CD163 for use in flow cytometry or other immunological applications like Western blot or immunohistochemistry.

Paraffin sections of human tonsils using 5C6-FAT

Frozen sections of human placenta using RM3/1

Fig.1: Paraffin sections of human tonsils using 5C6-FAT

Fig.2: Frozen sections of human placenta using RM3/1

Available antibody panel to CD163

CD163 antibody

References

Graverson, J. H. et al, Int J Biochem Cell Biol. 2002 Apr; 34(4):309-14.

Ritter, M. et al, Eur J Immunol. 2001 Apr; 31(4):999-1009

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD163 antibody

Staining of ED2 antibody on Rat spleen (frozen section) Cat.-No. BM4001 Mouse IgG1 ED2 Purified Rt C, F, ICC/IF, IP, P, WB
0.25 mg / €620.00
  Acris Antibodies GmbH

CD163 antibody

Staining of ED2 antibody on Rat spleen (frozen section) Cat.-No. BM4001 Mouse IgG1 ED2 Purified Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD163 antibody

BM4041 CD163 antibody staining of Paraffin Human Tonsil Section (Clone 5C6-FAT) Mouse IgG1 5C6-FAT Purified Hu C, E, F, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD163 antibody

BM4041B CD163 antibody staining of Frozen Human Placenta Section (Clone 5C6-FAT) Mouse IgG1 5C6-FAT Biotin Hu C, F, P
0.1 mg / €520.00
  Acris Antibodies GmbH

CD163 antibody

CD163 Mouse IgG1 5C6-FAT FITC Hu F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD163 antibody

Paraffin Sections of Human Tonsils using 5C6-FAT Mouse IgG1 5C6-FAT Purified Hu C, E, F, P, WB
1 mg / €1,020.00
  Acris Antibodies GmbH

CD163 (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta (4 µm) stained with anti-CD163 antibody (DB 045) shows strong positive cytoplasmic immunostaining of monocytes and macrophages. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG K20-T Aff - Purified Hu P
0.1 ml / €280.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD163 antibody

AM21062PU CD163 antibody staining of Human Lung. Rabbit IgG SP96 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

CD163 antibody

AM21062PU CD163 antibody staining of Human Lung. Rabbit IgG SP96 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

CD163 antibody

AM21062PU CD163 antibody staining of Human Lung. Rabbit IgG SP96 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD163 antibody

Staining of porcine peripheral blood mononuclear cells with FITC conjugated Mouse Anti Porcine CD163 antibody (AM05884FC-N) Mouse IgG1 2A10/11 FITC Por F
0.1 mg / €600.00
  Acris Antibodies GmbH

CD163 antibody

CD163 Mouse IgG1 ED2 Biotin Rt F
0.1 mg / €430.00
  Acris Antibodies GmbH

CD163 antibody

Staining of rat peritoneal macrophages with MOUSE ANTI RAT CD163: FITC Mouse IgG1 ED2 FITC Rt F
0.1 mg / €670.00
  Acris Antibodies GmbH

CD163 antibody

Staining of Rat peritoneal macrophages with Mouse anti Rat CD163 antibody RPE conjugated (Cat.-No BM4001R) Mouse IgG1 ED2 PE Rt F
100 Tests / €670.00
  Acris Antibodies GmbH

CD163 antibody

BM4021 CD163 antibody staining of Human Placenta Frozen Section. Mouse IgG1 RM3/1 Purified Hu, Mky C, F, WB
0.2 mg / €440.00
  Acris Antibodies GmbH

CD163 antibody

CD163 Mouse IgG1 Ki-M8 Purified Hu C, F, WB
0.1 mg / €530.00
  Acris Antibodies GmbH

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