Fibroblast Marker Antibodies - FocusOn 008

The main function of fibroblasts is to maintain the structural integrity of connective tissue by continuously secreting precursors of the extracellular matrix. Fibroblasts make collagens, glycosaminoglycans, reticular and elastic fibers and glycoproteins found in the extracellular matrix. Many researchers specifically stain fibroblasts to detect cell culture contamination. In the scientific literature several recommendations can be found to achieve this using antibodies to marker proteins specifically expressed by fibroblasts.

Prolyl-4-hydroxylase catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in peptide linkages. The active enzyme is a tetramer, consisting of two different types of inactive monomers with molecular weights of about 64 kDa (alpha subunit) and 60 kDa (beta subunit).

The monoclonal ER-TR7 antibody detects reticular fibroblasts, which constitute the cellular framework of lymphoid and non-lymphoid organs and their products. ER-TR7 reacts with an intracellular component of fibroblasts.

FSP1/S100A4 is a filament-associated calcium-binding protein and is associated with cells of mesenchymal origin or of fibroblastic phenotype. Polyclonal antisera raised to recombinant fibroblast specific protein 1 (FSP1) stain the cytoplasm of fibroblasts, but not epithelium. FSP1 is also expressed by many tumor cells spreading into distant metastases and is therefore also known as Metastasin.

Acris Antibodies presents a full panel of antibodies useful as fibroblast markers in human, mouse and rat tissues, including ER-TR7 antibody, Prolyl-4-hydroxylase antibodies and FSP1/S100A4 antibodies.



AMC staining of frozen mouse spleen section using rat anti mouse reticular fibroblast antibody ER-TR7 Cat.-No BM4018

AMC staining of frozen mouse spleen section using rat anti mouse reticular fibroblast antibody ER-TR7 Cat.-No BM4018

Staining of rat spleen paraffin section using mouse anti rat prolyl-4-hydroxylase beta antibody 6-9H6 Cat.-No. AF5110-1

Staining of rat spleen paraffin section using mouse anti rat prolyl-4-hydroxylase beta antibody 6-9H6 Cat.-No. AF5110-1

Staining of frozen rat skin section using mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody 6-9H6 Cat.-No. AF5110-1

Staining of frozen rat skin section using mouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody 6-9H6 Cat.-No. AF5110-1

Staining of human tonsil paraffin section using mouse anti human prolyl-4-hydroxylase beta (fibroblast marker) antibody 3-2B12 Cat.-No. AF0910-1

Staining of human tonsil paraffin section using mouse anti human prolyl-4-hydroxylase beta (fibroblast marker) antibody 3-2B12 Cat.-No. AF0910-1



Product Citations (Selection)

Catalog-No. Citations
SM1214P 1
AF0910-1 2
BM4018 3
AF5110-1 4
1
Michael R. Nazareth et al.: Characterization of human lungtumor-associated fibroblasts and their ability to modulate theactivation of tumor-associated T cells.
J. Immunol., May 2007; 178: 5552-5562.
2
Reinhart Seibl et al.: Expression and regulation of toll-like receptor 2 in rheumatoid arthritis synovium.
Am. J. Pathol., Apr 2003; 162: 1221-1227.
2
Beom K. Choi et al.: 4-1BB functions as a survival factor in dendritic cells.
J. Immunol., Apr 2009; 182: 4107-4115.
3
Ariel H. Achtman et al.: CCR7-deficient mice develop atypically persistent germinal centers in response to thymus-independent type 2 antigens.
J. Leukoc. Biol., Mar 2009; 85: 409-417.
3
Daniela Malan et al.: Lack of laminin 1 in ES cell-derived cardiomyocytes causes inhomogeneous electrical spreading despite intact differentiation and function.
Stem Cells, Jan 2009; 27: 88-99.
3
Javier Rangel-Moreno et al.: Pulmonary expression of CXC chemo-kine ligand 13, CC chemokine ligand 19, and CC chemokine ligand 21 is essential for local immunity to influenza.
PNAS, Jun 2007; 104: 10577-10582.
3
Javier Rangel-Moreno et. al.: Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue.
J. Immunol., Oct 2005; 175: 4904-4913.
4
Nadezda Koleganova et al.: Calcitriol ameliorates capillary deficitand fibrosis of the heart in subtotally nephrectomized rats.
Nephrol. Dial. Transplant., Mar 2009; 24: 778-787.
4
Wei Zheng et al.: Differential effects of cyclic and static stretch oncoronary microvascular endothelial cell receptors and vasculogenic/angiogenic responses.
Am. J. Physiol. Heart Circ. Physiol., Aug 2008; 295: H794-H800.
4
Navin K. Kapur et al.: Hemodynamic modulation of endocardial thromboresistance.
Circulation, Jan 2007; 115: 67-75.
4
Carol A. Podlasek et al.: Regulation of cavernous nerve injury-induced apoptosis by sonic hedgehog.
Biol. Reprod., Jan 2007; 76: 19-28.
4
Ina Gruh et al.: No evidence of transdifferentiation of human endothelial progenitor cells into cardiomyocytes after coculture with neonatal rat cardiomyocytes.
Circulation, Mar 2006; 113: 1326-1334.


Key References

2, 4
Yasuo Bai et al.: Immunological properties of monoclonalantibodies to human and rat prolyl 4-hydroxylase.
J. Biochem., 1986; 99: 1563-1570.
3
Els van Vliet et al.: Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody.
J. Histochem. Cytochem. 34: 883-890. (1986)


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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fibroblasts (Pan Reticular) antibody

Immunohistochemistry on Human Skin Frozen Sections using Fibroblast Antibody Cat.No BM4018 clone ER-TR7. Rat IgG2a ER-TR7 Purified Hu, Ms, Por C, F
0.2 mg / €500.00
  Acris Antibodies GmbH

Fibroblasts / Epithelial Cells antibody

Flow Cytometry: Staining of KG1 cells with Mouse anti Human fibroblast antibody. Mouse IgG2a D7-FIB Purified Hu C, F, IP
0.2 mg / €670.00
  Acris Antibodies GmbH

Fibroblasts / Epithelial Cells antibody

Flow Cytometry: Staining of KG1 cells with Mouse anti Human fibroblast antibody. Mouse IgG2a D7-FIB Purified Hu C, F, IP

Regular Price: 0.1 mg / €310.00

Special Price: 0.1 mg / €217.00

  Acris Antibodies GmbH

Prolyl 4-hydroxylase subunit beta antibody

Figure.1: AEC staining of Paraffin Sections of Human tonsills with Mouse Anti Human Prolyl-4-Hydroxylase beta (Fibroblast marker) clone 3-2B12. Cat.-No. AF0910-1 Mouse IgG1 3-2B12 Purified Hu C, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

Prolyl 4-hydroxylase subunit beta antibody

Figure 2. Staining of Rat spleen paraffin sections using Mouse anti Rat Prolyl-4-Hydroxylase beta (fibroblast marker) antibody 6-9H6 (Cat.-No AF5110-1). Mouse IgG1 6-9H6 Purified Rt C, E, ICC/IF, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

S100A4 antibody

AP15766PU S100A4 Antibody staining of Human Tonsil. Rabbit Purified Bov, Can, Hu, Ms, Rt P, WB
1 ml / €490.00
  Acris Antibodies GmbH

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