Organelle Markers: Antibodies to the Golgi Apparatus - FocusOn 119

Antibody panel useful as specific marker for the Golgi apparatus

Acris Antibodies offers a selections of antibodies useful as organelle markers. This FocusOn presents an antibody panel to proteins specific for the Golgi apparatus. These polyclonal and monoclonal antibodies can be used on different species and in most relevant immunological methods, like immunohistochemistry, immunoprecipitation or Western blotting (see ‘Product list’).

58K Golgi protein

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.

Fig. 1: <span>FTCD Antibody staining (AP16258PU-N at 3.8 µg/ml) of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.</span>
Fig. 1: FTCD Antibody staining (Cat.-No. AP16258PU-N at 3.8 µg/ml) of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. 
Fig. 2: <span>FTCD Antibody staining (AP16258PU-N at 0.03µg/ml) of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.</span>
Fig. 2: FTCD Antibody staining (Cat.-No. AP16258PU-N at 0.03µg/ml) of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 

TGOLN2/TGN46

Trans-Golgi network membrane protein 2 (TGOLN2) or TGN38 homolog is primary found in the Trans-Golgi Network (TGN). The TGN is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. It is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways. Isoform TGN46 seems to be widely expressed, whereas TGN51 is more abundant in fetal lung and kidney. TGN48 barely can be found in embryonic kidney and promyelocytic cells. TGOLN2 seems to be important in regulating the trafficking of proteins from the TGN to the cell surface.

Fig. 3: <span>Immunofluorescence staining of Human TGN46 in 3% paraformaldehyde-fixed HeLa cells using Affinity purified Sheep anti-Human TGN46 Antibody Cat.-No AP32693PU-N at 1/100. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488.</span>
Fig. 3: Immunofluorescence staining of Human TGN46 in 3% paraformaldehyde-fixed HeLa cells using Affinity purified Sheep anti-Human TGN46 Antibody Cat.-No AP32693PU-N at 1/100. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488.
Fig. 4: I<span>mmunofluorescence staining of TGN38 in 3% paraformaldehyde-fixed NRK cells using Sheep anti-Rat TGN38 Antibody Serum Cat.-No AP32691SU-N at 1/100 dilution. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488.</span>
Fig. 4: Immunofluorescence staining of TGN38 in 3% paraformaldehyde-fixed NRK cells using Sheep anti-Rat TGN38 Antibody Serum Cat.-No AP32691SU-N at 1/100 dilution. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488.

Mannosidase 2

Mannosidase 2 is a 135kD protein located on the luminal side of the Golgi membranes. It is useful as an immunocytochemical marker for the Golgi apparatus as well as for studying the fate of the Golgi complex in dividing cells.

Syntaxin 6

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled-coil domains and a carboxyl-terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans-Golgi network (TGN), and is partially co-localized with AP-1 on clathrincoated membranes. During secretory granule maturation, syntaxin 6 and MPR-ligand complexes are postulated to be transferred from immature granules to endosomes by clathrincoated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

GM130 (GOLGA2)

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localized to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure, and in vesicular transport. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Golgin-160 (GOLGA3)

The protein product of the GOLGA3 gene was designated golgin-160 based on its 160kDa molecular mass in mammalian cell extracts. Antibodies specific to golgin-160 show anti-Golgi reactivity by immunofluorescence; the Golgi staining is blocked by treatment with brefeldin A (BFA), a fungal antibiotic known to disrupt the stacked structure of the Golgi complex and to affect Golgi coatomer proteins.

Membrin (GS27)

Membrin, also known as GS27 (Golgi SNARE of 27K), is a 27kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport. GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, v-SNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: 0ne contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1. These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment.

COP

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta’-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Fig. 5:<span> Immunolocalization of COPI alpha in HeLa cells using SP5145P.</span>
Fig. 1:  Immunolocalization of COPI alpha in HeLa cells using (Cat.-No. SP5145P. 
Fig. 6: <span>Immunolocalization of beta-COP in NIH-3T3 cells using SP5139P.</span>
Fig. 2: Immunolocalization of beta-COP in NIH-3T3 cells using (Cat.-No. SP5139P.

Further readings

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Please refer also to our other FocusOn antibody panel for a comprehensive listing of our products for organelle markers:

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Syntaxin 6 / STX6 antibody

Western blot analysis: AM20177AF-N Syntaxin-6 antibody staining of Mouse brain (Lane 1), Rat brain (Lane 2), WR19L cells (Lane 3), PC12 cells (Lane 4). Mouse IgG1 3D10 Azide Free Hst, Ms, Rt ICC/IF, IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

TGN38 (Extracell. Dom) antibody

Rabbit anti-TGN38 Antibody Cat.-No AP32689PU-N on Raw 204.4 (Left), F(ab’)2 Goat anti rabbit IgG (H+L)-FITC conjugated secondary antibody (R1410F, 1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS. Rabbit Serum Ms, Rt EM, ICC/IF, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

TGN38 (Extracell. Dom) antibody

Immunofluorescence staining of TGN38 in 3% paraformaldehyde-fixed NRK cells using Sheep anti-Rat TGN38 Antibody Serum Cat.-No AP32691SU-N at 1/100 dilution. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488. Sheep Serum Ms, Rt C, ICC/IF, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

TGOLN2 / TGN46 antibody

Immunofluorescence staining of Human TGN46 in 3% paraformaldehyde fixed HeLa cells using Rabbit anti-Human TGN46 Antibody Serum Cat.-No AP32690SU-N at 1/100. Secondary antibody was Goat anti-Rabbit Alexa Fluor 488. Rabbit Serum Hu, Prim EM, ICC/IF, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

TGOLN2 / TGN46 antibody

Immunofluorescence staining of Human TGN46 in 3% paraformaldehyde-fixed HeLa cells using Affinity purified Sheep anti-Human TGN46 Antibody Cat.-No AP32693PU-N at 1/100. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488. Sheep IgG Purified Hu, Prim EM, ICC/IF, WB
0.1 ml / €350.00
  Acris Antibodies GmbH

TGOLN2 / TGN46 antibody

Immunofluorescence staining of Hhuman TGN46 in 3% paraformaldehyde-fixed HeLa cells using Sheep anti-Human TGN46 Antibody Serum Cat.-No AP32693SU-N at 1/100. Secondary antibody was Donkey anti-Sheep Alexa Fluor 488. Sheep Serum Hu, Prim C, ICC/IF, IP, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

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