Antibodies to NRAMP1/2, MTP1 and Dcytb - FocusOn 026

Introduction: NRAMP1/2, MTP1 and Dcytb are Natural Resistance and Iron Metabolism Proteins

CYBRD1 antibody Cat.-No AP32023PU-N staining of paraffin embedded Human Testis at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
CYBRD1 antibody Cat.-No. AP32023PU-N staining of paraffin embedded Human Testis at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis: AP17748PU-N NRAMP1 Antibody staining of CEM cell line lysates (35 µg/lane). NRAMP1 (arrow) was detected using the purified Pab.
Western blot analysis: Cat.-No. AP17748PU-N NRAMP1 Antibody staining of CEM cell line lysates (35 µg/lane). NRAMP1 (arrow) was detected using the purified Pab.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Cat.-No. AM31134PU-N.
AP08318PU-N MTP1 antibody staining of Formalin-Fixed Paraffin Embedded Human Skeletal Muscle.
Cat.-No. AP08318PU-N MTP1 antibody staining of Formalin-Fixed Paraffin Embedded Human Skeletal Muscle.

NRAMP1

Natural resistance to infection with unrelated intracellular parasites such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Naturally occurring and experimentally induced mutations at Nramp1 abrogate natural resistance to infection. Polymorphic variants at the human NRAMP1 locus are associated with differential susceptibility of humans to tuberculosis and leprosy. NRAMP1 is expressed in the lysosomal compartment of monocytes and macrophages and in lung and spleen. It is induced in response to lymphokines and bacterial products. After phagocytosis, Nramp1 is targeted to the membrane of the microbe-containing phagosome, where it may modify the intraphagosomal milieu to affect microbial infection. Although the physiological role of Nramp1 is unclear, structural predictions suggest that Nramp1 encodes the prototypic member of a transporter family. NRAMP1 (human 550 aa, chromosome 2q35; mouse 548 aa) is a highly hydrophobic integral membrane glycoprotein composed of 12-transmembrane (TM) domains that possess several structural characteristics of ion channels and transporters. Both N and C-termini are predicted to be cytoplasmic.

NRAMP2

Recently a second member of the NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal Ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. However, NRAMP2 expression is more pronounced in brain, thymus, proximal intestine, kidney, and bone marrow. It is dramatically up regulated by iron starvation in the intestine. The NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3\' exons encoding distinct C-termini of the protein as well as distinct 3\' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3\'UTR. The IRE is an RNA secondary structure present in the 5\'- or the 3\'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) is replaced by a novel 25-aa segment and codes for a distinct 3\' UTR lacking the IRE. The two isoforms are differentially localized and regulated in gastrointestinal tract and kidney. It has been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgrade (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

MTP1

More recently a novel iron-regulated gene that is homologous to the DMT1, termed Metal Transporter Protein (MTP1, human 571 aa, mouse 570 aa; ~90% similarity), has been identified. MTP1 is also described as iron-regulated transporter (IREG1) and Ferroportin1. MTP1 contains IRE in the 5\'-UTR. It is expressed in tissues involved in body iron homeostasis including the developing and mature reticuloendothelial system, the duodenum, and the pregnant uterus. MTP1 is also expressed in muscle and embryonic brain. In the adult mouse, MTP1 expression in the liver and duodenum are reciprocally regulated. Iron deficiency induces MTP1 expression in the duodenum but down-regulates expression in the liver. Therefore, MTP1 may be involved in intracellular iron metabolism.

Dcytb

Iron is an essential element for various physiological processes. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A number of ferric reductases have been identified in yeast, plants, and bacteria but the identity of such proteins remained unknown in mammals. Most recently, a new gene called Dcytb (for duodenal cytochrome b) has been cloned and characterized in mouse duodenum that may function as a mammalian ferric reductase.

Dcytb (human 286 aa, rat 290 aa) is a 6-transmembrane domain protein with ~45% sequence identity with cytochrome b561 reductase. However, no sequence homology exists with plant and yeast Dcytb. The N-terminus of mouse Dcytb is almost identical with the rabbit\'s p30 protein called cytochrome b558. Putative binding sites for cytochrome b561 substrates (ascorbic acid and semidehydroascorbic acid) are partially conserved in Dcytb and b561. Unlike most proteins involved in iron metabolism, Dcytb gene lacks a definable iron-responsive element. Dcytb is highly expressed in the brush border membrane of duodenal entrecotes.

Antibody Tools for the Detection of NRAMP1/2, MTP1 and Dcytb

Acris Antibodies offers a full panel to NRAMP and related proteins. These antibodies are available in various formats and can be used in immunological methods like Western blotting, ELISA or immunohistochemistry.



Available Antibody Panel to Natural Resistance and Iron Metabolism Proteins
NRAMP1 antibody, NRAMP2 antibody, MTP1 (Metal Transporter Protein) antibody, Dcytb (Duodenal cytochrome b) antibody



3 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CYBRD1 antibody


CYBRD1 antibody Cat.-No AP32023PU-N staining of paraffin embedded Human Testis at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Aff - Purified Bov, Can, Hu, Por P
0.1 mg / €340.00
  Acris Antibodies GmbH

MTP1 (N-term) antibody

Figure 1. AP08318PU-N MTP1 antibody staining of Formalin-Fixed Paraffin Embedded Human Skeletal Muscle. Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NRAMP2 / SLC11A2 (1-66) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4C6 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

3 Item(s)

per page
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