MCH and MCH Receptor - FocusOn 009

Introduction

Melanin-concentrating hormone (MCH) is a cyclic neuropeptide (human/rat 19 aa) that regulates a variety of functions in mammalian brain, in particular feeding behavior. MCH is synthesized in mainly in the lateral hypothalamus and zona incerta. MCH stimulates feeding, its level is unregulated in the hypothalamus of genetically obese mice, and mice lacking MCH eat less and are lean. MCH is thought to influence feeding and energy balance by acting downstream of leptin and the melanocortin system.

MCH Receptor type 1 (MCH-R1) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors. It is primarily expressed in the ventromedial and dorsomedial nuclei of the hypothalamus. MCH-RI binds Melanin-concentrating hormone with sub-nanomolar affinity, and is stimulated by MCH to mobilize intracellular Ca and reduce forskolin-elevated cAMP levels.

MCH Receptor type 2 (MCH-R2) shares considerable homology with MCH-R1. Like MCH-R1, it binds MCH with high affinity. However, reports indicate that signaling through this receptor is pertussis insensitive, contrary to MCH-R1.

Antibody Tools for Detection of Melanin Concentrating Hormone and MCH-Receptor

Acris Antibodies offers a panel of polyclonal antibodies to MCH, MCH-Receptor 1 and MCH-Receptor 2 (MCHR1 and MCHR2) for use in immunohistochemistry or other immunological detection methods like ELISA or Western blot.

Fig. 1: <span>Immunohistochemical staining of Brain using anti- MCHR1 antibody SP4317P.</span>
Fig. 1: Immunohistochemical staining of Brain using anti- MCHR1 antibody Cat.-No. SP4317P.
Fig. 2: <span>Frozen sections of mouse brain stained with MCHR2 antibody (BP4005).</span>
Fig. 2: Frozen sections of mouse brain stained with MCHR2 antibody. Cat.-No. BP4005.
Fig. 3: <span>Western blot analysis of MCHR1 / GPR24 Antibody (C-term) Cat.-No AP52635PU-N in CEM,K562 cell line lysates (35ug/lane). This demonstrates the MCHR1 antibody detected the MCHR1 protein (arrow).</span>
Fig. 3: Western blot analysis of MCHR1 / GPR24 Antibody (C-term) Cat.-No. AP52635PU-N in CEM,K562 cell line lysates (35ug/lane). This demonstrates the MCHR1 antibody detected the MCHR1 protein (arrow).

Available antibody panel to MCH and MCH Receptor

Melanin-concentrating hormone (MCH), Melanin Concentrating Hormone Receptor Type I ( MCH Receptor 1 / MCH-RI / MCHRI / MCHR1), Melanin Concentrating Hormone Receptor Type II antibody ( MCH Receptor 2 / MCH-RII / MCHRII / MCHR2).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCHR1 / GPR24 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with MCHR1 / GPR24 Antibody (C-term) Cat.-No AP52635PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MCHR2 / GPR145 antibody

Western blot (WB) analysis of MCH-2R antibody (Cat.-No.: AP01217PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MCHR2 / GPR145 antibody

Frozen sections of mouse brain stained with MCHR2 antibody (BP4005). Chicken Aff - Purified Hu, Ms, Rt C
0.2 mg / €500.00
  Acris Antibodies GmbH

MCHR2 / GPR145 (C-term) antibody

Immunohistochemical staining of Brain (Neurons) using anti- MCHR2 antibody SP4319P Rabbit Aff - Purified Hu, Mky, Prim ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

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