Metabotropic Glutamate Receptor Antibodies - FocusOn 108

Introduction: An mGluR is an GPCR activated by Glutamate

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on ligand-gated receptor channels but recently, glutamate has been shown to play another role as activator of G-protein-coupled receptors (GPCRs) called metabotropic glutamate receptors (mGluR). Based on sequence similarity, transduction mechanisms and agonist potencies, mGluRs are subdivided into three groups:

Group I:           mGluR1 / mGluR5
Group II:          mGluR2 / mGluR3
Group III:         mGluR4 / mGluR6 / mGluR7 / mGluR8

In expression systems, Group-I receptors, stimulate phospho-lipase C and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. mGluRs are widely distributed throughout the nervous system and are expressed by both neurons and glial cells. They have been suggested to play a variety of functional roles, among which is involvement in synaptic plasticity underlying learning and memory as well as chronic pain.

Antibody Tools for the Detection of mGluR1-8

Table 1 shows Acris Antibiodies outstanding antibody panel to mGluR subtypes. Most of these antibodies are suitable for immunohistochemistry and many have been proven to work on paraffin sections. A majority of these antibodies has also been tested for Western blot and immunofluorescence applications.



Immunohistochemistry on human brain sections (pituitary) using mGluR3 antibody SP4400P

Immunohistochemistry on human brain sections (neurons and glia) using mGluR8 antibody SP4405P

Fig.1: Immunohistochemistry on human brain sections (pituitary) using mGluR3 antibody SP4400P

Fig.2: Immunohistochemistry on human brain sections (neurons and glia) using mGluR8 antibody SP4405P

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Fig.3: Immunofluorescence on rat brain section using mGluR1 alpha antibody RA19065-100 (red; blue: DAPI counterstain)



Literature

Enz R. The trick of the tail: protein-protein interactions of metabotropic glutamate receptors. Bioessays. 2007 Jan; 29(1): 60-73.

Available Antibody Panel to mGluR subtypes

All mGluR antibody products: mGluR1 antibody, mGluR2 antibody, mGluR3 antibody, mGluR4 antibody, mGluR5 antibody, mGluR6 antibody, mGluR7 antibody, mGluR8 antibody.



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glutamate receptor 1 / GLUR1 pSer831 antibody

Figure 1.Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser831 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser831 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Can, Chk, Hu, Ms, Rt WB
0.15 ml / €380.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer845 antibody

Figure 1. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Hu, Ms, non-Human Primates, Rt ICC/IF, WB
0.15 ml / €380.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer849 antibody

Western blot analysis of extract from mouse brain tissue, using GluR1 antibody (Lane 1 and 2) and GluR1 (pSer849) antibody (AP02510PU-S; AP02510PU-N, Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer849 antibody

Western blot analysis of extract from mouse brain tissue, using GluR1 antibody (Lane 1 and 2) and GluR1 (pSer849) antibody (AP02510PU-S; AP02510PU-N, Lane 3 and 4). Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 antibody

Western blot analysis of extracts from C6 and PC12 using GluR1 antibody (AP02755PU-S; AP02755PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 antibody

Western blot analysis of extracts from C6 and PC12 using GluR1 antibody (AP02755PU-S; AP02755PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer831 antibody

Western blot analysis using GluR1 Ser831 antibody on rat brain lysate in the absence and presence of antigen peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, WB
10 Blots / €460.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer845 antibody

Western blot analysis using GluR1 Ser845 antibody on rat brain lysate in the absence and presence of antigen peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
10 Blots / €460.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 (C-term) antibody

Glutamate receptor 1 / GLUR1 Rabbit IgG Purified Hst, Hu, Mky, Rt C, WB
50 µg / €400.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer831 antibody

Glutamate receptor 1 / GLUR1 Rabbit IgG Purified Can, Chk, Hu, Ms, Rt WB
0.15 ml / €510.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 pSer845 antibody

Glutamate receptor 1 / GLUR1 Rabbit IgG Purified Hu, Mky, Ms, Rt WB
0.15 ml / €510.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using GluR1 Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €270.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 antibody

Immunofluorescence staining of methanol-fixed HeLa cells using GluR1 Antibody Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
50 µl / €230.00
  Acris Antibodies GmbH

Glutamate receptor 1 / GLUR1 antibody

Western blot analysis of extracts from mouse brain tissue , using GRIA1 polyclonal antibody ( Cat # PAB5371 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Glutamate receptor 1 / GLUR1 antibody

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k Gria1 protein phosphorylated at Ser831 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Gria1 (phospho S831) polyclonal antibody ( Cat # PAB9661 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit Chk, Hu, Ms, Rt WB
0.15 ml / €510.00
  Abnova Taiwan Corp.

Glutamate receptor 1 / GLUR1 antibody

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k Gria1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Gria1 (phospho S845) polyclonal antibody ( Cat # PAB9662 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit Hu, Ms, Rt WB
0.15 ml / €510.00
  Abnova Taiwan Corp.

Glutamate receptor 1 / GLUR1 antibody

Immunohistochemical staining of GRIA1 on formalin fixed, paraffin embedded human brain with GRIA1 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Glutamate receptor 1 / GLUR1 (894-907) antibody

Staining with GluR1 (Ionotropic Glutamate Receptor 1) Antibody (24439) Rabbit Serum Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €430.00
  ImmunoStar Inc.

Glutamate receptor 1 / GLUR1 Control Peptide

Glutamate receptor 1 / GLUR1 Purified
25 µg / €190.00
  ImmunoStar Inc.

Glutamate receptor 1 / GLUR1 antibody

Immunohistochemistry: GluR1 Antibody (E308) [NB110-57005] - Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-GluR1 (catalog #110-57005). Rabbit IgG E308 Aff - Purified Hu IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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