NK-Cell Markers - FocusOn 114

Natural Killer cells (NK cells) are cytotoxic effector cells, that carry out surveillance and self defense functions while detecting and destroying hazardous targets like autologous tumor cells and pathogen-infected cells. Research in this area during the last years has identified a couple of proteins that may prove useful as markers for NK cells.

KIR

KIR (Killer cell Immunoglobulin (Ig)-like Receptor) designate a family of surface proteins, that are expressed on NK cells and some T-cells. The subgroup of the KIR2D family (2D =  2 Ig-domains) amongst others has the following members: KIR2DL1 (CD158a), KIR2DL3 (CD158b), KIR2DL4 (CD158d) and KIR2DS4 (p50.3).

LAIR

LAIR-1 is member of the family of Leukocyte-associated Ig like Receptors. It is a transmembrane glycoprotein, that is expressed on f.e. T-cells, B-cells, NK-cells, macrophages and dendritic cells. The Activation and crosslinking of LAIR-1 molecules on NK-cells results in strong inhibition of NK-cell mediated cytotoxicity.

NCR (NKp’s)

The family of Natural Cytotoxicity Receptors (NCR) is comprised of Ig-like receptors, that play an important role in activation of NK-cells in conjunction with tumor cell defense. NKp30, the Natural Cytotoxicity triggering Receptor 3 (NCR3) f.e., cooperates with NKp46 and NKp44 in inducing cytotoxicity against a multitude of target cells and is selectively expressed by all NK-cells. Therefore NKp30 is an optimal marker for identification of NK-cells.

Antibodies and Proteins

The table below shows proteins and antibodies for use as NK-cell markers. Some antibodies are available in different conjugated formats, which makes them suitable for varying methods.

Review:

(1)
Farag, SS, Fehniger, TA, Ruggeri, L, Velardi, A, Caligiuri, MA (2002) Natural killer cell receptors: new biology and insights into the graft-versus-leukemia effect. Blood 100: 1935 - 47.


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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD8 antibody

CD8 Mouse IgG1 ANK61 Supernatant Rt C, F, IP, P
1 ml / €530.00
  Acris Antibodies GmbH

CD8 antibody

CD8 Mouse IgG1 ANK44 Supernatant Rt C, F, IP
1 ml / €530.00
  Acris Antibodies GmbH

CD158a / KIR2DL1 antibody

Western blot analysis: Recombinant Human protein KIR2DL1, KIR2DL3, KIR2DS4 and KIR2DL4 (each 50 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6033 anti-Human KIR2DL1 antibody (1/500). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 2F9 Purified Hu E, IP, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD158a / KIR2DL1 antibody

Western blot analysis: Recombinant Human protein KIR2DL1, KIR2DL3, KIR2DS4 and KIR2DL4 (each 50 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6033 anti-Human KIR2DL1 antibody (1/500). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a 2F9 Purified Hu E, IP, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD158b / KIR2DL3 antibody

CD158b / KIR2DL3 Mouse IgG1 GL183 Purified Hu F, IP, WB
0.2 mg / €530.00
  Acris Antibodies GmbH

CD158b / KIR2DL3 antibody

CD158b / KIR2DL3 Mouse IgG2a KIR2DL3 Purified Hu F, WB
0.2 mg / €600.00
  Acris Antibodies GmbH

CD158b / KIR2DL3 antibody

CD158b / KIR2DL3 Mouse IgG2a KIR2DL3 PE Hu F
100 Tests / €590.00
  Acris Antibodies GmbH

CD158b / KIR2DL3 antibody

Profile of NK cells analyzed by Flow Cytometry Mouse IgG2a 190IIC311 Purified Hu E, F, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

CD158d / KIR2DL4 antibody

CD158d / KIR2DL4 Mouse IgG1 mAb#33 Aff - Purified Hu F, FN, ICC/IF, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

CD158d / KIR2DL4 antibody

CD158d / KIR2DL4 Mouse IgG1 mAb#33 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD158d / KIR2DL4 (L1, L3, L4, S4) antibody

Western blot analysis: Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6021 anti-Human KIR2DL4 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.  
Lane 1 : Extracellular domain of KIR2DL1(23-223aa) 
Lane 2 : Extracellular domain of KIR2DL3(23-223aa) 
Lane 3 : Extracellular domain of KIR2DL4(44-202aa) Mouse IgG2b 2H6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD158d / KIR2DL4 (L1, L3, L4, S4) antibody

Western blot analysis: Human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6021 anti-Human KIR2DL4 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.  
Lane 1 : Extracellular domain of KIR2DL1(23-223aa) 
Lane 2 : Extracellular domain of KIR2DL3(23-223aa) 
Lane 3 : Extracellular domain of KIR2DL4(44-202aa) Mouse IgG2b 2H6 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD158i / KIR2DS4 antibody

Western blot analysis Recombinant Human KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4 (each 100 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6023 anti-Human KIR2DS4 antibody (1/1000). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates recombinant Human KIR2DS4 protein. Mouse IgG2b 5F2 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD158i / KIR2DS4 antibody

Western blot analysis Recombinant Human KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4 (each 100 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6023 anti-Human KIR2DS4 antibody (1/1000). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates recombinant Human KIR2DS4 protein. Mouse IgG2b 5F2 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

CD158 / KIR2D Mouse IgG1 NKVFS1 FITC Hu F
0.1 mg / €420.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

CD158 / KIR2D Mouse IgG1 NKVFS1 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

CD158 / KIR2D Mouse IgG1 NKVFS1 Low Endotoxin Hu E, F, FN, IP, WB
0.5 mg / €570.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

Staining of Human peripheral blood lymphocytes with Mouse Anti Human KIR Antibody (Cat.-No SM2036P). Mouse IgG1 NKVFS1 Aff - Purified Hu E, F, IP, WB
0.2 mg / €490.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

Staining of human peripheral blood lymphocytes with mouse anti-human KIR/KIR2D antibody Cat.-No. SM2036PS. Mouse IgG1 NKVFS1 Aff - Purified Hu E, F, IP, WB
0.1 mg / €280.00
  Acris Antibodies GmbH

CD158 / KIR2D antibody

Staining of human peripheral blood lymphocytes with mouse anti-human KIR/KIR2D antibody Cat.-No. SM2036PS. Mouse IgG1 NKVFS1 Aff - Purified Hu E, F, IP, WB
25 µg / €180.00
  Acris Antibodies GmbH

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