Antibodies to nuclear RNPs - FocusOn 030

Introduction: There exist two groups of ribonucleoproteins in the nucleus: the small nuclear ribonucleoproteins (snRNP´s) and the heterogenous nuclear Ribonucleopreins (hnRNP´s).

In the nucleoplasm, the U1 snRNP along with other small nuclear ribonucleoproteins (U2, U4-U6, and U5) assemble into splicosomes that remove introns from pre-mRNA by splicing. The U1 snRNA forms base pairs with conserved sequence motifs at the 5\'-splice site and recognizes both the 5\'- and 3\'-splice sites and may have a fundamental role in aligning the two sites for the splicing reaction. The U2 snRNA forms base pairs with conserved sequence motifs at the branch point, which associates with a heat- and RNAase-sensitive factor in an early step of splicing.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm (hnRNP A1 and hnRNP K, f. e.). The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The 120 kDa hnRNP particle U is an abundant nucleoplasmic phosphoprotein and the largest of the major hnRNP proteins. hnRNP U is specifically involved in pre-mRNA processing and is directly bound to both RNA and DNA. hnRNP M is involved in pre-mRNA splicing and in stress-induced transient splicing arrest whereas hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA.

Antibody Tools for the Detection of Ribonucleoproteins

Acris Antibodies offers a panel of antibodies to snRNP and hnRNP which can be used for immunological detection methods like western blotting, immunoprecipitation, or immunocytochemistry .



Immunocytochemical detection of hnRNP-A2/B1 on acetone fixed HEp-II cells with AM26527AF-N.
 
Western blot analysis of hnRNP-A2/B1 expression in Jurkat (1), Raji (2), HeLa (3), MCF-7 (4) and NIH/3T3 (5) using AM26527AF-N.
Fig.1: Antibody Cat.-No. AM26527AF-N used for (left image) immunocytochemical detection of hnRNP-A2/B1 on acetone fixed HEp2 cells and (right image) Westernblot analysis of hnRNP-A2/B1 expression in Jurkat (lane 1), Raji (lane 2), HeLa (lane 3), MCF-7 (lane 4) and NIH/3T3 (lane 5) cells.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma reacted with HNRNPC Antibody (C-term) Cat.-No AP52068PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Formalin-Fixed, Paraffin-Embedded Human Colon stained with anti-hnRNPK Antibody Cat.-No AM06025PU (Clone F45P9C7) at 15 µg/ml after heat-induced antigen retrieval.
Fig.2: Immunohistochemistry analysis in formalin fixed, paraffin embedded human bladder carcinoma reacted with HNRNPC-antibody Cat.-No. AP52068PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fig.3: Immunohistochemical detection of hnRNPK in formalin fixed, paraffin embedded human colon using antibody Cat.-No. AM06025PU-M at 15 µg/ml after heat-induced antigen retrieval.


Available Antibody Panel to RNP ’s
RNP antibody, hnRNP-A1 (hnRNP A1), -A2/B1, -C1/C2, -F, -F/H, -H,- H1, - K, -K/J, -L, -M1-M4, -Q and hnRNP-U antibody, U1 snRNP 70 kD antibody, U1 sn RNP A, A/B’’ and U2 snRNP B’’ antibody



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

hnRNP-A2/B1 / HNRNPA2B1 antibody

Immunocytochemical detection of hnRNP-A2/B1 on acetone fixed HEp-II cells with AM26527AF-N. Mouse IgM C20308 Azide Free Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

hnRNP-C1/C2 / HNRNPC (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma reacted with HNRNPC Antibody (C-term) Cat.-No AP52068PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HNRNPCL1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue reacted with HNRNPCL1 Antibody (C-term) Cat.-No AP52069PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

hnRNP-H / HNRNPH1 antibody

Immunohistochemistry analyzes of hnRNP H antibody (Cat.-No.: AP20424PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

hnRNP-H / HNRNPH1 (C-term) antibody

hnRNP-H / HNRNPH1 Rabbit Aff - Purified Hu IP, WB
50 µg / €330.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK antibody

AM05384PU-N hnRNP-K antibody staining of Formalin-Fixed, Paraffin-Embedded Normal Colon (Left) and Colon
Carninoma (Right). Mouse IgG1 F45 P9 C7 Purified Chk, Hu, Ms, Rb, Rt C, WB
0.2 mg / €340.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK antibody

Western blot (WB) analysis of hnRNP K antibody (Cat.-No.: AP01359PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK (N-term) antibody

(LEFT) Western blot analysis of anti-HNRPK Antibody (N-term) (Cat.#AP12520PU-N) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-HNRPK Antibody (N-term) (Cat.#AP12520PU-N) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

hnRNP-L / HNRNPL antibody

(LEFT) Western blot analysis of HNRPL Antibody (Center) (AP18050PU-N) in HepG2, Jurkat cell line lysates (35ug/lane). HNRPL (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of HNRPL (arrow) using rabbit polyclonal HNRPL Antibody (Center) (AP18050PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2) . Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

hnRNP-L / HNRNPL (C-term) antibody

(LEFT) Western blot analysis of HNRPL Antibody (C-term) (AP18051PU-N) in HepG2 cell line lysates (35ug/lane). HNRPL (arrow) was detected using the purified Pab.(2ug/ml) (RIGHT) Western blot analysis of HNRPL (arrow) using rabbit polyclonal HNRPL Antibody (C-term) (AP18051PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HNRPL gene (Lane 2) . Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

hnRNP-M / HNRNPM antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3F7 Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

hnRNP-M / HNRNPM antibody

Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using hnRNP-M / HNRNPM antibody (Cat.-No. AP01281PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

hnRNP-Q / HNRPQ (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with HNRPQ antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

U1 snRNP protein A antibody

Human Small Intestine, submucosal plexus: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3F9-1F7 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

U1 snRNP protein A (Center) antibody

SNRPA Antibody (Center) (Cat. #AP53964PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the SNRPA antibody detected the SNRPA protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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