Antibodies to Nup153 - FocusOn 032

Introduction: Transportin 1 (TRN1) acts as the import receptor for shuttling hnRNP proteins with an M9 nuclear localization sequence (NLS). Searching for proteins that interact with TRN1 a short region within the N-terminus of the nucleoporin Nup153 which binds TRN1 was indentified.

Nup153 is located at the nucleoplasmic face of the nuclear pore complex (NPC), in the distal basket structure, and functions in mRNA export. It was shown that this Nup153 TRN1-interacting region is an M9 NLS. Both import and export receptors interact with several regions of Nup153, in a RanGTP-regulated fashion. RanGTP dissociates Nup153-import receptor complexes, but is required for Nup153-export receptor interactions. Nup153 is a RanGDP-binding protein, and the interaction is mediated by the zinc finger region of Nup153. This represents a novel Ran-binding domain, which was termed the zinc finger Ran-binding motif. Nup153 shuttles between the nuclear and cytoplasmic faces of the NPC. The presence of an M9 shuttling domain in Nup153, together with its ability to move within the NPC and to interact with export receptors, suggests that this nucleoporin is a mobile component of the pore which carries export cargos towards the cytoplasm.

Antibody Tools for the Detection of Nup153

OriGene Technologies offers a monoclonal and two polyclonal antibodies to Nup153. These antibodies can be used for immunofluorescence / immunohistochemistry, flow cytometry, immunoblotting & immunoprecipitation.

Confocal microscope analysis of Nup153, lamin B3 and F/GXFG nucleoporin distribution

Confocal microscope analysis of Nup153, lamin B3 and F/GXFG nucleoporin distribution in sperm pronuclei


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Available Antibody
Nup153 antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUP153 (N-term) antibody

Western blot analysis of NUP153 Antibody (N-term) Cat.-No AP52970PU-N in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, WB
0.4 ml / €415.00
  OriGene Technologies GmbH

NUP153 antibody

NUP153 Mouse IgG1 nup7A8 Supernatant Amph, Hu, Ms, Rt, Xen C, ICC/IF, IP, WB
5 ml / €300.00
  OriGene Technologies GmbH

2 Item(s)

per page
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