Antibodies to Ubiquitin - FocusOn 025

Introduction: Ubiquitin regulates the protein turnover in a cell.

Ubiquitin is a small, 76-residue protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein\'s function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction.

Ubiquitin+1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially ub+1 is accumulated in aggregates containing amyloid-beta and phosphorylated-tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer\'s disease. Also ub +1 acts as an aggravating factor in polyglutamine induced neurodegeneration.

Antibody Tools for the Detection of Ubiquitin

Acris Antibodies offers a wide range of antibodies for the detection of Ubiquitin. These antibodies are available in various formats and can be used in immunological methods like Western blotting, ELISA or immunohistochemistry.

Fig. 1: Immunohistochemical staining of Ubiquitin in Formalin fixed, Paraffin processed tissue of Human hypophysis using antibody SM1422P.
Fig. 1: Immunohistochemical staining of Ubiquitin in Formalin fixed, Paraffin processed tissue of Human hypophysis using antibody Cat.-No. SM1422P.
Fig. 2: Immunohistochemical staining of Formalin-Fixed Paraffin-Embedded human testing tissue using plyclonal anti-Ubiquitin+1 antibody AP23462PU-N.
Fig. 2: Immunohistochemical staining of Formalin-Fixed Paraffin-Embedded human testing tissue using plyclonal anti-Ubiquitin+1 antibody Cat.-No. AP23462PU-N.
Fig. 3: Flow cytometry analysis of Ubiquitin in HeLa cells using monoclonal anti-Ubiquitin antibody Cat.-No. AM06722PU-N (green) and negative control (red).
Fig. 3: Flow cytometry analysis of Ubiquitin in HeLa cells using monoclonal anti-Ubiquitin antibody Cat.-No. AM06722PU-N (green) and negative control (red).
Fig. 4: Western blot analysis using monoclonal anti-Ubiquitin antibody AM06722PU-N against NIH/3T3 (lane 1) and HeLa (lane 2) cell lysate.
Fig. 4: Western blot analysis using monoclonal anti-Ubiquitin antibody Cat.-No. AM06722PU-N against NIH/3T3 (lane 1) and HeLa (lane 2) cell lysate.
Fig. 5: Anti-Ubiquitin antibody Cat.-No. R1199 was tested by immunoblot against total cell extract from yeast. A 1:500 dilution of the antibody showed strong reactivity with Ubiquitinated proteins. The antibody was incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche).
Fig. 5: Anti-Ubiquitin antibody Cat.-No. R1199 was tested by immunoblot against total cell extract from yeast. A 1:500 dilution of the antibody showed strong reactivity with Ubiquitinated proteins. The antibody was incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche).
 

Available Antibody Panel to Ubiquitin

Ubiquitin antibody, Ubiquitin+1

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Polyubiquitin-C / UBC (C-term) antibody

Immunohistochemistry: AP11230PU-N Ubiquitin antibody staining of Formalin-Fixed, Paraffin-Embedded Human cancer tissue, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu ICC/IF, P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

Ubiquitin+1 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Ubiquitin (N-term) antibody

Western Blot. 5 μg of recombinant protein from Phytophora sp. (Lane 1), human (Lane 2), Arabidopsis thaliana His-tagged ubiquitin (Lane 3), Arabidopsis thaliana His-tagged SUMO protein (Lane 4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1/10000 anti-Ubiquitin antibody (1h), followed by incubation with 1/15000 secondary anti-Rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL. Rabbit Serum Arabidopsis thaliana, Hu, Pl WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Ubiquitin antibody

Western blot analysis using UBB antibody Cat.-No AM06722PU-N against human UBB (AA: 1-299) recombinant protein (Expected MW is 26 kDa). Mouse IgG1 3C12 Purified Hu E, F, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Ubiquitin (N-term) antibody

Immunohistochemistry: AP11229PU-N  Ubiquitin antibody staining of Formalin-Fixed, Paraffin-Embedded Human hepatocarcinoma tissue which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

Ubiquitin antibody

Immunohistochemistry: AP15846PU-N Ubiquitin antibody staining of Human Alzheimer's brain. Rabbit Purified Hu P
0.1 ml / €170.00
  Acris Antibodies GmbH

Ubiquitin antibody

Western blot analysis of Ubiquitin in human cell lysates using a 1:1000 dilution of the antibody Mouse IgG2a 5B9-B3 Purified Bov, Hu, Ms, Rt E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Ubiquitin antibody

Western blot analysis of Ubiquitin in human cell lines using a 1:1000 dilution of the antibody Mouse IgG2a 6C11-B3 Purified Bov, Hu, Ms, Rt E, WB
50 µg / €230.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Mouse IgG1 Ubi-1 Br C, ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Mouse IgG1 Ubi-1-AP Purified Hu ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Ubiquitin antibody

Western blot analysis of ubiquitin expression in Raji (1), free ubiquitin (2) and PPUb4* (3) using AM26659AF-N. *PPUb4;partially purified multi-ubiquitin chains in ubiquitin-protein conjugates Mouse IgG1 1B3 Azide Free Bov, Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Mouse IgG1 2C5 Purified Bov, Hu, Ms, Rt WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Rabbit Purified Bov ID, WB
1 ml / €330.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Rabbit IgG Purified Bov, Eq, Hu, Ms, Rt C, ICC/IF, P
1 ml / €320.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin Mouse IgG1 242.9 Ascites Hu C, P, WB
0.1 ml / €610.00
  Acris Antibodies GmbH

Ubiquitin antibody

Ubiquitin - SM1422P - Human hypophysis. Formalin fixed, paraffin processed tissue. Mouse IgG1 1B4-UB Purified Bov, Hu C, E, P, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

16 Item(s)

per page
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