Antibodies to Programmed Death-1 (PD-1) and its Ligands PD-L1 and PD-L2 - FocusOn 019

Introduction

Protein PD-1 (programmed death-1, also known as CD279, gene name PDCD1) is a 55 KDa member of the immunoglobulin superfamily. It contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.

Two members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.

PD-L1 (B7H1), a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. PD-L1 is expressed on a majority of leukocytes. Interaction of PD-1 with either PDL1 or PDL2 results in inhibition of T and B cell responses.

PD-L2 (B7DC) a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and it also belongs to the Ig superfamily. PD-L2 is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although PD-L2 has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation.



Fig. 1: AP07695PU-N CD274 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus.
Fig. 1: AP07695PU-NCD274 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus.
Fig. 2: AP07336PU-N PDCD1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine.
Fig. 2: AP07336PU-NPDCD1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine.
Fig. 3: Immunohistochemistry of PDL-2 in mouse brain tissue with AP30656PU-N at 2.5 μg/ml.
Fig. 3: Immunohistochemistry of PDL-2 in mouse brain tissue with AP30656PU-N at 2.5 μg/ml.
Fig. 4: Small intestine, Peyer's patch: Formalin-Fixed, Paraffin-Embedded (FFPE)
Fig. 4: Small intestine, Peyer's patch: Formalin-Fixed, Paraffin-Embedded (FFPE). Cat.-No. AP16366PU-N


Antibody Tools for the Detection of Programmed Death-1 and its Ligands PDL1 (B7H1) and PDL2 (B7DC)

Available antibody panel to PD-1, PD-L1 and PD-L2

PD-1 antibody ( PDCD1 antibody, CD279 antibody), PD-L1 (PDL1 , B7-H1 , CD274 ), PD-L2 (PDL2 , B7-DC , CD273 )
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD273 / PDL2 antibody

CD273 / PDL2 Rat IgG2a TY25 FITC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD273 / PDL2 antibody

CD273 / PDL2 Rat IgG2a TY25 Purified Ms C, F, IP
0.25 mg / €400.00
  Acris Antibodies GmbH

CD273 / PDL2 antibody

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD273:FITC AM05616FC-N). Mouse IgG1 MIH14 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD273 / PDL2 antibody

CD273 / PDL2 Mouse IgG1 MIH14 Purified Hu C, F
0.2 mg / €480.00
  Acris Antibodies GmbH

CD273 / PDL2 antibody

CD273 / PDL2 Mouse IgG1 MIH14 Purified Hu C, F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD273 / PDL2 antibody

CD273 / PDL2 Mouse IgG1 MIH14 PE Hu F
100 Tests / €470.00
  Acris Antibodies GmbH

CD273 / PDL2 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with CD273 / PDL2 Antibody (N-term) Cat.-No AP53228PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of Mouse peripheral blood lymphocytes with Rat anti Mouse CD274 (Cat.-No AM05612PU-N) Rat IgG2a MIH6 Purified Ms C, F, IP
0.1 mg / €240.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Immunohistochemical detection of CD274 on human 10 weeks placenta paraffin embedded section with AM26531AF-N. Mouse IgG2b 27A2 Azide Free Hu F, P
0.1 mg / €360.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of Mouse peripheral blood lymphocytes with Rat anti Mouse CD274 antibody -FITC (AM05612FC-N) Rat IgG2a MIH6 FITC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD274 Antibody Low Endotoxin (Cat.-No AM05612LE-N) Rat IgG2a MIH6 Low Endotoxin Ms C, F, IP
0.5 mg / €560.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

CD274 / PDL1 Rat IgG2a MIH6 Purified Ms C, F, IP
0.25 mg / €390.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Flow Cytometry: AM05613FC-N FITC conjugated CD274 antibody Staining of Human peripheral blood monocytes. Mouse IgG1 MIH2 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274(AM05613PU-L). Mouse IgG1 MIH2 Purified Hu F
0.2 mg / €490.00
  Acris Antibodies GmbH

CD274 / PDL1 antibody

Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274(AM05613PU-L). Mouse IgG1 MIH2 Purified Hu F
0.1 mg / €290.00
  Acris Antibodies GmbH

CD279 / PD1 antibody

AP23806PU CD279 antibody staining of Human Tonsil. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD279 / PD1 (21-167) antibody

Cell lysates of 293T and U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b 4F12 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD279 / PD1 antibody

CD279 / PD1 Rat IgG2b RMP1-30 Purified Ms F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD279 / PD1 antibody

CD279 / PD1 Rat IgG2a RMP1-14 Purified Ms C, F
0.25 mg / €390.00
  Acris Antibodies GmbH

CD279 / PD1 antibody

CD279 / PD1 Mouse IgG1 MIH4 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

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