Antibodies to Programmed Death-1 (PD-1) and its Ligands PD-L1 and PD-L2 - FocusOn 019

Introduction

Protein PD-1 (programmed death-1, also known as CD279, gene name PDCD1) is a 55 KDa member of the immunoglobulin superfamily. It contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes.

Two members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.

PD-L1 (B7H1), a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. PD-L1 is expressed on a majority of leukocytes. Interaction of PD-1 with either PDL1 or PDL2 results in inhibition of T and B cell responses.

PD-L2 (B7DC) a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and it also belongs to the Ig superfamily. PD-L2 is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although PD-L2 has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation.



Fig. 1: AP07695PU-N CD274 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus.
Fig. 1: AP07695PU-NCD274 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus.
Fig. 2: AP07336PU-N PDCD1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine.
Fig. 2: AP07336PU-NPDCD1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small Intestine.
Fig. 3: Immunohistochemistry of PDL-2 in mouse brain tissue with AP30656PU-N at 2.5 μg/ml.
Fig. 3: Immunohistochemistry of PDL-2 in mouse brain tissue with AP30656PU-N at 2.5 μg/ml.
Fig. 4: Small intestine, Peyer's patch: Formalin-Fixed, Paraffin-Embedded (FFPE)
Fig. 4: Small intestine, Peyer's patch: Formalin-Fixed, Paraffin-Embedded (FFPE). Cat.-No. AP16366PU-N


Antibody Tools for the Detection of Programmed Death-1 and its Ligands PDL1 (B7H1) and PDL2 (B7DC)

Available antibody panel to PD-1, PD-L1 and PD-L2

PD-1 antibody ( PDCD1 antibody, CD279 antibody), PD-L1 (PDL1 , B7-H1 , CD274 ), PD-L2 (PDL2 , B7-DC , CD273 )

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD273 / PDL2 antibody

CD273 / PDL2 Rat IgG2a TY25 Purified Ms C, F, IP
0.25 mg / €460.00
  OriGene Technologies GmbH

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