Regulators of G-Protein Signaling - FocusOn 111

The Regulators of G-Protein Signaling (RGS) play a key role in the regulation of signal transduction by GPCRs (G-Protein coupled Receptors). They represent a protein family, who share a characteristic RGS domain. By this domain RGS proteins bind directly to activated G-alpha subunits and so function as GAPs  (GTPase−activating Proteins). Most RGS proteins are able to function as GAPs for different isoforms of G-alpha and so have influence on multiple signaling pathways. RGS proteins may however also show extreme specificity towards single signaling pathways.

RGS proteins may be regulated in their GAP activity or in their subcellular distribution. This is done by phopsphorylation, palmitoylation or through interaction with protein or lipid ligands. Many RGS proteins have GAP-independent functions, which influence the GPCR- and non-GPCR-mediated signal transduction, and so function as effector regulator or as effector itself. For this reason RGS proteins can be thought of as multifunctional regulators of signal transduction.

Antibodies to RGS

Acris Antibodies offers a large panel of antibodies for detection of RGS proteins in Western blot. Many antibodies are also reactive with RGS from mouse and rat and some have been tested successfully on paraffin sections (see pictures below).



Fig. 1a:  RGS6 Staining IHC(P) on human kidney tissue using RGS6 antibody ARP34043. Arrows indicate RGS6-positive epithelial cells of renal tubuli.Fig. 1b:  RGS13 Staining IHC(P) on human kidney tissue using RGS13 antibody ARP34051. Arrows indicate RGS13 positive epithelial cells.
Fig. 1a:  RGS6 Staining IHC(P) on human kidney tissue using RGS6 antibody ARP34043. Arrows indicate RGS6-positive epithelial cells of renal tubuli.

Fig. 1b:  RGS13 Staining IHC(P) on human kidney tissue using RGS13 antibody ARP34051. Arrows indicate RGS13 positive epithelial cells.



Literature

Hendriks-Balk MC, Peters SL, Michel MC, Alewijnse AE. Regulation of G protein-coupled receptor signalling: focus on the cardiovascular system and regulator of G protein signalling proteins. Eur J Pharmacol. 2008 May 13;585(2-3):278-91.

Jean-Baptiste G, Yang Z, Greenwood MT. Regulatory mechanisms involved in modulating RGS function. Cell Mol Life Sci. 2006 Sep;63(17):1969-85.

Available Antibody Panel for Regulators of G-Protein Signalling

Anti- RGS1 (1R20, BL34), RGS2 (G0S8), RGS3 (RGP3), RGS4 (RGP4), RGS5, RGS6 (S914), RGS7, RGS8, RGS9, RGS10, RGS11, RGS12, RGS13, RGS14, RGS16 (RGSR), RGS17, RGS18 (RGS13), RGS19 (GAIP, GNAI3IP), RGS20 (RGSZ1, ZGAP1) -antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RGS2 antibody

Immunohistochemistry staining of human brain / cortex (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using RGS2 antibody Cat.-No. AP07203PU-N (concentration 20 ug/ml). Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RGS2 antibody

Mouse SP2/0; WB Suggested Anti-Rgs2 Antibody Titration: 1.25ug/ml. Positive Control: SP2/0 cell lysate; Rgs2 antibody - middle region (ARP42827_T100) in Mouse SP2/0 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

RGS2 antibody

RGS2 Chicken Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €490.00
  Novus Biologicals Inc.

RGS3 antibody

Human Heart; WB Suggested Anti-RGS3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart; RGS3 antibody - C-terminal region (AVARP09007_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

RGS3 antibody

HepG2; Host:. Rabbit. Target Name:. RGS3. Sample Tissue:. HepG2 lysates. Antibody Dilution: 1.0ug/ml; RGS3 antibody - C-terminal region (AVARP09007_T100) in HepG2 cells using Western Blot Rabbit Aff - Purified Chk, Hu, Ms, Rt
0.1 mg / €310.00
  AVIVA Systems Biology

RGS3 antibody

Western Blot: RGS3 Antibody [NB120-2564] - Western blot analysis of  RGS3 in transfected HEK293  cells (right lane). The left  lane is mock control. Rabbit IgG Aff - Purified Hu IP, WB
0.1 mg / €470.00
  Novus Biologicals Inc.

RGS3 antibody

RGS3 Rabbit Purified Hu IP, WB
0.2 mg / €370.00
  Torrey Pines Biolabs

RGS4 antibody

Human Jurkat; WB Suggested Anti-RGS4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; RGS4 antibody - C-terminal region (ARP30213_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RGS4 antibody

Western Blot: E coli-derived fusion protein as test antigen. Affi-anti-RGS4 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS4 ) Chicken Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €490.00
  Novus Biologicals Inc.

RGS6 antibody

Human kidney; RGS6 antibody - C-terminal region (ARP34043_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

RGS6 antibody

Western Blot: RGS6 Antibody [NB100-56659] - Western blot analysis of RGS6 in 30 ugs of mouse brain tissue lysate using NB100-56659 at 1:1000 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.2 ml / €320.00
  Novus Biologicals Inc.

RGS6 antibody

Western Blot: E coli-derived fusion protein as test antigen. Affi-anti- RGS6 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS6 ) Chicken Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €520.00
  Novus Biologicals Inc.

RGS7 antibody

Mouse NIH-3T3; WB Suggested Anti-Rgs7 Antibody Titration: 5.0ug/ml. Positive Control: NIH/3T3 cell lysate; Rgs7 antibody - N-terminal region (ARP42839_T100) in Mouse NIH-3T3 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

RGS8 antibody

Human HepG2; WB Suggested Anti-RGS8 Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; RGS8 antibody - N-terminal region (ARP42803_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

RGS9 antibody

Human Liver; RGS9 antibody - N-terminal region (AVARP09024_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

RGS10 antibody

Human Jurkat; WB Suggested Anti-RGS10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that RGS10 is expressed in Jurkat.; RGS10 antibody - middle region (AVARP09013_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Hu, Por F, WB
50 µg / €350.00
  AVIVA Systems Biology

RGS10 antibody

Human Jurkat; WB Suggested Anti-RGS10 Antibody. . Titration: 1.25 ug/ml. . Positive Control: Jurkat Whole Cell. There is BioGPS gene expression data showing that RGS10 is expressed in Jurkat.; RGS10 antibody - middle region (AVARP09013_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

RGS10 antibody

RGS10 Chicken Aff - Purified Hu WB
50 µg / €520.00
  Novus Biologicals Inc.

RGS12 antibody

Western Blot: E coli-derived fusion protein as test antigen. Affi-anti- RGS12b IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS12 ) Chicken Aff - Purified Hu, Ms, Rt WB
50 µg / €520.00
  Novus Biologicals Inc.

RGS13 antibody

Human Daudi; WB Suggested Anti-RGS13 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. RGS13 is supported by BioGPS gene expression data to be expressed in Daudi.; RGS13 antibody - middle region (ARP34051_P050) in Human Daudi cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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