Stem Cell Markers: Antibodies to Embryonic Stem Cells - FocusOn 106

Embryonic stem cells are derived from the inner cell mass (ICM) of the blastocyst. They can proliferate indefinitely in vitro (self renewal) and differentiate into cells of all three germ layers (pluripotency).

Sox1 and Sox2

SOX1 and 2 belong to the Sox (Sry-related HMG-box) family of transcription factors. Having about 20 members in multiple species, the Sox family is characterized by the DNA-binding HMG domain. Sox proteins are involved in different developmental processes like embryonic development and determination of cell fate. Sox2, Oct3/4 and Nanog are a self-stabilizing network of transcription factors which maintain the stem cell phenotype of human and murine stem cells. Sox2 belongs to the structurally similar group B with their members Sox1 and Sox3, showing overlapping expression in the fetal nervous sytem, suggested to have similar functions.

Oct3/4

Oct3/4 is also known as POU5F1. This protein is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct3/4 is required to sustain stem-cell-renewal, and up- or downregulation induces divergent developmental programmes.

Fig. 1: Immunofluorescence analysis of Sox2 antibody Cat.-No. AP11920PU-N in HeLa cells
Fig. 1: Immunofluorescence analysis of Sox2 antibody Cat.-No. AP11920PU-N in HeLa cells
Fig. 2: Formalin-fixed and paraffin-embedded (FFPE) human lung carcinoma tissue reacted with Sox2 antibody (N-term) Cat.-No. AP11920PU-N; peroxidase-conjugated secondary antibody / DAB staining
Fig. 2: Formalin-fixed and paraffin-embedded (FFPE) human lung carcinoma tissue reacted with Sox2 antibody (N-term) Cat.-No. AP11920PU-N; peroxidase-conjugated secondary antibody / DAB staining
Fig. 3: Human newborn brain stained with Sox2 antibody Cat.-No. AM09112PU-N
Fig. 3: Human newborn brain stained with Sox2 antibody Cat.-No. AM09112PU-N
Fig. 4: Human prostate (FFPE) stained with Oct3/4 antibody Cat.-No. AP07188PU-N
Fig. 4: Human prostate (FFPE) stained with Oct3/4 antibody Cat.-No. AP07188PU-N

Nanog

The homeobox transcription factor Nanog is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem and embryonic germ cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway.

SSEA

Stage-specific embyronic antigen-1 (SSEA-1) is expressed on murine and human germ cells, murine embryonic stem cells and murine embryonal carcinoma cells. SSEA-4, a glycolipid carbohydrate epitope, is a widely used marker to monitor the differentiation of pluripotent embryonic stem cells.

Fig. 5: Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with Nanog antibody Cat.-No. AP16396PU-N (green staining). Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain
Fig. 5: Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with Nanog antibody Cat.-No. AP16396PU-N (green staining). Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain
Fig. 6: Staining of human uterus (FFPE) with SSEA-1 antibody Cat.-No. AM08357PU-N; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen
Fig. 6: Staining of human uterus (FFPE) with SSEA-1 antibody Cat.-No. AM08357PU-N; biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen

Frizzled-5

Frizzled proteins (Frizzled; FZD) are 7-transmembrane receptors which act as receptors for Wnt proteins. At present, at least ten different human Frizzled genes have been described, playing essential roles in the regulation of embryonic development, tissue and cell polarity, formation of neural synapses and the regulation of proliferation. The signalling pathway has been shown to be implicated in the genesis of numerous human cancers.

E-cadherin

E-cadherin, a calcium dependent cell adhesion molecule, is expressed predominately in epithelial tissues and plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. It is concluded that E-cadherin protein stabilizes cortical actin cytoskeletal arrangement in ES cells (Spencer, 2007).

Fig. 7: Detection of endogenous E-cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with E-cadherin antibody clone 22F8 Cat.-No. AM00010PU-N: Lane 1: A431; Lane 2: SW480; Lane 3: SW620; Lane 4: HT29; Lane 5: MCF-7; Lane 6: MDA-MB 231; Lane 7: T47D
Fig. 7: Detection of endogenous E-cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with E-cadherin antibody clone 22F8 Cat.-No. AM00010PU-N: Lane 1: A431; Lane 2: SW480; Lane 3: SW620; Lane 4: HT29; Lane 5: MCF-7; Lane 6: MDA-MB 231; Lane 7: T47D
Fig. 8: Human skin (FFPE) stained with E-cadherin antibody Cat.-No. AP15381PU-S
Fig. 8: Human skin (FFPE) stained with E-cadherin antibody Cat.-No. AP15381PU-S

HMGA2

The HMGA2 protein (High Mobility Group family) is expressed exclusively during mammalian embryonic development and in some cancers. It belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. The expression of HMGA2 is high in neural stem cells and decreases with age.

References

Bowles J et al: Phylogeny of the Sox family of developmental transcription factors based on sequence and structural indicators.
Dev Biol, Nov 2000; 227(2):239-55. [PMID 11071752 ]
Pan G et al: Nanog and transcriptional networks in embryonic stem cell pluripotency.
Cell Res, Jan 2007; 17(1): 42-9. [PMID 17211451 ]
Schulz WA et al: Transcription factor networks in embryonic stem cells and testicular cancer and the definition of epigenetics.
Epigenetics, Jan 2007; 2(1): 37-42. [PMID 17965618 ]
Spencer HL et al: E-cadherin inhibits cell surface localization of the pro-migratory 5T4 oncofetal antigen in mouse embryonic stem cells.
Mol Biol Cell, Aug 2007; 18(8): 2838-51. [PMID 17507657 ]
Wiebe MS et al: Identification of novel domains within Sox2 and Sox11 involved in autoinhibition of DNA binding and partnership specificity.
J Biol Chem, May 2003; 278(20): 17901-11. [PMID 12637543 ]
Ye F et al: Stage-specific embryonic antigen 4 expression in epithelial ovarian carcinoma.
Int J Gynecol Cancer, Aug 2010; 20(6): 958-64. [PMID 20683402 ]

Further readings

See the PDF-version: FocusOn 106

Please refer also to our other FocusOn antibody panel for a comprehensive listing of our products for stem cell research

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD15 antibody

CD15 Mouse IgM Bu28 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD15 antibody

CD15 Mouse IgM Bu28 Purified Hu F
0.2 mg / €440.00
  Acris Antibodies GmbH

CD15 antibody

CD15 Mouse IgM Bu28 Purified Hu F
25 µg / €180.00
  Acris Antibodies GmbH

CD15 antibody

AM08357PU-N FUT4 Antibody staining of Formalin-Fixed Paraffin-Embedded Human Uterus at 1/50 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgM HI98 Purified Hu C, F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 (600-707) antibody

Western Blot analysis of MCF7 cell lysate with E-cadherin antibody Rabbit IgG SP64 Purified Hu P, WB
1 ml / €750.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 (600-707) antibody

Western Blot analysis of MCF7 cell lysate with E-cadherin antibody Rabbit IgG SP64 Purified Hu P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 antibody

Human breast stained with anti-cadherin-E/E-cadherin antibody Mouse IgG2b SPM471 Purified Hu P
1 ml / €500.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 antibody

Human breast stained with anti-cadherin-E/E-cadherin antibody Mouse IgG2b SPM471 Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 antibody

AP15381PU Cadherin E antibody staining of Human Skin. Rabbit Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 antibody

AP15381PU Cadherin E antibody staining of Human Skin. Rabbit Purified Hu P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 (incl. pos. control) antibody

CD324 / Cadherin-1 Mouse IgG1 22F8 Biotin Hu WB
0.1 mg / €490.00
  Acris Antibodies GmbH

CD324 / Cadherin-1 (incl. pos. control) antibody

Detection of endogenous E - cadherin
Whole cell lysates of serum starved tumor cells (20.000 cells
per lane) were applied to SDS-PAGE and transferred to a
PVDF membrane. The immunoblot was probed with mab
22F8 (0.5 μg/ ml) for 1h at RT and developed by ECL
(exp. time: 30 sec).
lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29;
lane 5: MCF-7; lane 6: MDA-MB 231; lane 7: T47D Mouse IgG1 22F8 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

FZD5 / Frizzled-5 (N-term, Extracell. Dom.) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Can, Elephant, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

NANOG (1-154) antibody

Immunohistochemistry: Paraffin Embedded Sections of Human seminoma tissue were incubated with anti-Human Nanog (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG2a 5A10 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

NANOG antibody

Immunohistochemistry: Paraffin Embedded Sections of Human seminoma tissue were incubated with anti-Human Nanog (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG2a 5A10 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

NANOG antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using anti-NANOG monoclonal antibody Cat.-No AM11049SU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated. Mouse IgG1 60CT70.5.12 Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15694PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15694PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

OCT3/4 antibody

Formalin fixed paraffin embedded Testicular Seminoma stained with OCT4 antibody Cat.-No AM03173PU (Clone NRG1.1) Mouse IgG2b NRG1.1 Purified Hu P
1 ml / €610.00
  Acris Antibodies GmbH

OCT3/4 antibody

Formalin fixed paraffin embedded Testicular Seminoma stained with OCT4 antibody Cat.-No AM03173PU (Clone NRG1.1) Mouse IgG2b NRG1.1 Purified Hu P
0.5 ml / €410.00
  Acris Antibodies GmbH

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