Stem Cell Markers: Antibodies to Hematopoietic Stem Cells - FocusOn 054

Introduction

Multipotent stem cells, hemangioblasts, are derived from the mesoderm and differentiate toward hematopoietic and endothelial cell lineages. Hematopoietic stem cells (HSCs) can be found in the bone marrow, peripheral blood, and umbilical cord blood. HSCs are multipotent stem cells that give rise to all the blood cell types from the myeloid (monocytes and macrophages, neutrophils, basophils, eosinophils, erythrocytes, megakaryocytes/platelets, dendritic cells), and lymphoid lineages (T-cells, B-cells, NK-cells). HSCs are the most clinically used stem cell population and are used to treat hematopoietic malignancies and disorders.

Two populations of hematopoietic stem cells have been identified, long term and short term. Long term HSCs are capable of self renewal. Short term HSCs, also called progenitor or precursor cells, can differentiate into all types of blood cell but do not have the capacity for self renewal. These properties of HSCs are used in transplant therapies to treat leukemia and congenital immunodeficiency disorders.

Hematopoietic stem cells represent approximately 1 in 10,000 cells of the bone marrow and 1 in 100,000 cells in the blood. In addition, hematopoietic stem cells are morphologically very similar to white blood cells. Thus, isolation and identification of HSCs are dependent on the detection of cell surface markers and cluster of differentiation antigens. HSCs are commonly characterized by the absence of lineage-specific marker expression. Human hematopoietic stem cells are determined as CD34+, CD59+, CD90+, CD38low/–, c-Kit–/low and Lin–. Mouse hematopoietic stem cells are considered CD34low/–, SCA-1+, CD90+/low, CD38+, c-Kit+ and Lin–.

CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

CD38

CD38 is a type II transmembrane protein that functions as a signaling molecule and mediates the adhesion between lymphocytes and endothelial cells. It also functions enzymatically in the formation and hydrolyzation of the second messenger cyclic ADP ribose. In the hematopoietic system, CD38 is most highly expressed on plasma cells.

Fig. 1: Formalin-fixed, paraffin-embedded (FFPE) human placenta tissue stained with CD34 antibodiy Cat.-No. AM08318PU-N
Fig. 1: Formalin-fixed, paraffin-embedded (FFPE) human placenta tissue stained with CD34 antibodiy Cat.-No. AM08318PU-N
Fig. 2: Spleen (FFPE) stained with CD38 antibody Cat.-No. AM20475SU-N
Fig. 2: Spleen (FFPE) stained with CD38 antibody Cat.-No. AM20475SU-N

CD59

CD59 is a GPI-anchored membrane glycoprotein that functions to inhibit formation of the complement membrane attack complex and thereby prevents cell injury. CD59 is expressed on erythrocytes and on all peripheral blood leukocytes. In mouse, the gene encoding CD59 has been duplicated, giving rise to CD59a and CD59b. The two proteins are differentially expressed in tissues and share approximately 65 % amino acid sequence identity.

Fig. 3: Skin (pilosebaceous unit; FFPE) stained with CD59 antibody Cat.-No. AM08314PU-N
Fig. 3: Skin (pilosebaceous unit; FFPE) stained with CD59 antibody Cat.-No. AM08314PU-N
Fig. 4: Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL using CD59 antibody (clone MEM-43/5) Cat.-No. SM3033P: Lane 1: original cell lysate; Lane 2: material immunoprecipitated with CD59 antibody (clone MEM-43) Cat.-No. SM1145PS
Fig. 4: Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL using CD59 antibody (clone MEM-43/5) Cat.-No. SM3033P: Lane 1: original cell lysate; Lane 2: material immunoprecipitated with CD59 antibody (clone MEM-43) Cat.-No. SM1145PS

CD90

CD90, also known as Thy1, is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation.

c-Kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Fig. 5: Human kidney (FFPE) stained with CD90 antibody Cat.-No. AM20160PU-N
Fig. 5: Human kidney (FFPE) stained with CD90 antibody Cat.-No. AM20160PU-N
Fig. 6: Human gastrointestinal stroma tumor stained with c-kit ligand / CD117 antibody Cat.-No. AM11098PU-N
Fig. 6: Human gastrointestinal stroma tumor stained with c-kit ligand / CD117 antibody Cat.-No. AM11098PU-N

SCA-1

SCA-1 is an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family. SCA-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSCs in mice of both Ly-6 haplotypes. SCA-1-positive HSCs can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal. However, SCA-1 has also been discovered in several non-hematopoietic tissues and can be used to enrich other progenitor cell populations as well. It is suggested that SCA-1 could be involved in regulating both B and T cell activation.

Further readings

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD34 antibody

Staining of CLL-1390 cells with Mouse anti Canine CD34:RPE (Cat.-No SM2257R). Mouse IgG1 1H6 PE Can F
100 Tests / €590.00
  Acris Antibodies GmbH

CD34 Class II antibody

Human lung stained with anti-CD34 antibody Mouse IgG1 QBEND/10 Purified Hu P
1 ml / €510.00
  Acris Antibodies GmbH

CD34 Class II antibody

Human lung stained with anti-CD34 antibody Mouse IgG1 QBEND/10 Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD34 (Endothelial Cell Marker) antibody

Human angiosarcoma stained with anti-CD34 antibody Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD34 (Endothelial Cell Marker) antibody

Human angiosarcoma stained with anti-CD34 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD34 antibody

Immunohistochemistry analysis of Formalin-Fixed Paraffin-Embedded Human Placenta tissue, using AM08318PU-N at 10 µg/ml. Mouse IgG1 4H11[APG] Purified Hu F, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD34 antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) using anti CD34 antibody (Cat#AM08428PU-N) Rat IgG2a MEC 14.7 Purified Hu, Ms F, IP, P
50 µg / €470.00
  Acris Antibodies GmbH

CD34 antibody

CD34 Mouse IgG1 34C02 Purified Hu C, F
50 µg / €240.00
  Acris Antibodies GmbH

CD34 antibody

Staining of mouse bone marrow cells with Rat Anti Mouse CD34-FITC (SM1603F) Rat IgG2a MEC14.7 FITC Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD34 antibody

Staining of mouse bone marrow cells with Rat Anti Mouse CD34-FITC (SM1603F) Rat IgG2a MEC14.7 FITC Ms F
50 µg / €270.00
  Acris Antibodies GmbH

CD34 antibody

Staining of mouse bone marrow cells with Rat Anti Mouse CD34-FITC (SM1603F) Rat IgG2a MEC14.7 FITC Ms F
25 µg / €190.00
  Acris Antibodies GmbH

CD34 antibody

CD34 Rat IgG2a MEC14.7 FITC Ms F
0.5 mg / €560.00
  Acris Antibodies GmbH

CD34 antibody

CD34 Rat IgG2a MEC14.7 Low Endotoxin Ms F, IP, P
0.5 mg / €540.00
  Acris Antibodies GmbH

CD34 antibody

Staining of WEHI cells with Rat anti Mouse CD34 (SM1603P/PS/PT). Rat IgG2a MEC14.7 Purified Ms F, IP
0.25 mg / €390.00
  Acris Antibodies GmbH

CD34 antibody

Staining of WEHI cells with Rat anti Mouse CD34 (SM1603P/PS/PT). Rat IgG2a MEC14.7 Purified Ms F, IP
0.1 mg / €240.00
  Acris Antibodies GmbH

CD34 antibody

Staining of WEHI cells with Rat anti Mouse CD34 (SM1603P/PS/PT). Rat IgG2a MEC14.7 Purified Ms F, IP
25 µg / €170.00
  Acris Antibodies GmbH

CD34 antibody

Staining of WEHI cells with Rat Anti-Mouse CD34 antibody -RPE conjugated. Rat IgG2a MEC14.7 PE Ms F
100 Tests / €290.00
  Acris Antibodies GmbH

CD34 antibody

Staining of WEHI cells with Rat Anti-Mouse CD34 antibody -RPE conjugated. Rat IgG2a MEC14.7 PE Ms F
25 Tests / €210.00
  Acris Antibodies GmbH

CD34 antibody

CD34 Mouse IgG1 1H6 FITC Can F
0.1 mg / €520.00
  Acris Antibodies GmbH

CD38 antibody

Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE. Mouse IgG1 HIT2 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

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