Stem Cell Markers: Antibodies to Mesenchymal Stem Cells - FocusOn 053

Introduction

Mesenchymal Stem Cells (MSCs), also known as Marrow Stromal Cells, are multipotent stem cells found in the stroma of non-hematopoietic bone marrow. MSCs have a large capacity for self-renewal while maintaining their multipotency . MSCs and MSC-like cells have now been isolated from various sites other than the bone marrow, including adipose tissue, amniotic fluid, periosteum, and fetal tissues, and show phenotypic heterogeneity. MSC-like cells have been isolated from pathological tissues such as the rheumatoid arthritic joint, and these cells express bone morphogenetic protein receptors. Indeed, it has been suggested that cells with mesenchymal stem characteristics reside in virtually all postnatal organs and tissues. In a steady state of cell turnover, or in response to trauma, MSCs are induced to differentiate into a wide array of cells types including osteoblasts, chondrocytes, myocytes, adipocytes, beta-pancreatic islet cells and possibly neuronal cells.

Markers expressed by mesenchymal stem cells include CD105 (SH2), CD73 (SH3/4), CD44, CD90 (Thy-1), CD71 and Stro-1 as well as the adhesion molecules CD106, CD166, and CD29. Among negative markers for mesenchymal stem cells (not expressed) are hematopoietic markers CD45, CD34, CD14, CD11 and the costimulatory molecules CD80, CD86 and CD40 as well as the adhesion molecules CD31, CD18 and CD56.

CD105

Endoglin, also known as CD105, is a type I integral transmembrane glycoprotein and is an accessory receptor for TGF-beta superfamily ligands. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages.

Fig. 1: Formalin-fixed paraffin-embedded (FFPE) prostate tissue (vessel) stained with CD105 antibody Cat.-No. AP08365PU-N
Fig. 1: Formalin-fixed paraffin-embedded (FFPE) prostate tissue (vessel) stained with CD105 antibody Cat.-No. AP08365PU-N
Fig. 2: Staining of KG1 cells with CD105 antibody Cat.-No. SM1177P
Fig. 2: Staining of KG1 cells with CD105 antibody Cat.-No. SM1177P

CD73

CD73, an ecto-5’-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that forms a disulfide-linked homodimer. The 5’-nucleotidase activity of CD73 converts extracellular nucleoside 5’-monophosphates to nucleosides. It is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. Most cell types express CD73.

CD44

CD44 is an 80 to 250 kDa type I transmembrane glycoprotein that binds hyaluronan and a variety of extracellular as well as cell surface ligands. The molecule exists in multiple spliced forms and shows enormous variability in glycosylation.

Fig. 3: Human liver (FFPE) stained with CD73 antibody Cat.-No. AM20411SU-N
Fig. 3: Human liver (FFPE) stained with CD73 antibody Cat.-No. AM20411SU-N
Fig. 4: Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263) Cat.-No. SM3024P; Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL
Fig. 4: Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263) Cat.-No. SM3024P; Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

CD90

CD90, also known as Thy1, is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation.

CD106

Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes such as macrophages. VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and Integrin alpha 4 beta 7.

CD166

Activated leukocyte cell adhesion molecule (ALCAM) is a type I membrane glycoprotein that is a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat.

Fig. 5: Immunofluorescent staining: BALB/c mesenteric lymph node cells were stained with either rat IgG2c-FITC (as a negative control) or rat anti-mouse CD90-FITC Cat.-No. AM08054FC-N. Lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA)
Fig. 5: Immunofluorescent staining: BALB/c mesenteric lymph node cells were stained with either rat IgG2c-FITC (as a negative control) or rat anti-mouse CD90-FITC Cat.-No. AM08054FC-N. Lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD44 antibody

Human Esophageal carcinoma stained with Anti-CD44 antibody Mouse IgG2a SPM521 Purified Hu P, WB
1 ml / €500.00
  Acris Antibodies GmbH

CD44 antibody

Human Esophageal carcinoma stained with Anti-CD44 antibody Mouse IgG2a SPM521 Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

CD44 antibody

Human esophageal carcinoma stained with anti-CD44 antibody Rabbit IgG SP37 Purified Bov, Can, Hu P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

CD44 antibody

Human Esophageal carcinoma stained With anti-CD44 Rabbit IgG Aff - Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD44 antibody

Human Esophageal carcinoma stained With anti-CD44 Rabbit IgG Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD44 (21-145) antibody

Immunohistochemistry: AM03178PU-N Cd44 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 5C10 Purified Hu E, P, WB
0.1 ml / €260.00
  Acris Antibodies GmbH

CD44 (21-145) antibody

Immunohistochemistry: AM03178PU-N Cd44 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b 5C10 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2b IM7 FITC Can, Eq, Fe, Hu, Ms
0.1 mg / €200.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2b IM7 Purified Can, Eq, Fe, Hu, Ms C, F, ICC/IF, IP, P, WB
0.1 mg / €160.00
  Acris Antibodies GmbH

CD44 control peptide

CD44 Purified
50 µg / €190.00
  Acris Antibodies GmbH

CD44 antibody

Western blot analysis of HCAM/CD44 using mouse small intestine tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 Azide Free Ms C, F
1 mg / €620.00
  Acris Antibodies GmbH

CD44 antibody

Flow Cytometry - Cell Source: Spleen - Percentage of cells stained above control: 92.5% Rat IgG2a KM81 Biotin Ms C, F
0.1 mg / €230.00
  Acris Antibodies GmbH

CD44 antibody

Flow Cytometry - Cell Source: Spleen - Percentage of cells stained above control: 92.5% Rat IgG2a KM81 Biotin Ms C, F
0.3 mg / €400.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 FITC Ms F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 FITC Ms F
0.3 mg / €400.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 Purified Ms C, F
0.25 mg / €310.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 PE Ms F
50 µg / €230.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Rat IgG2a KM81 PE Ms F
0.3 mg / €540.00
  Acris Antibodies GmbH

CD44 antibody

CD44 Mouse IgG2a OX-49 Ascites Rt C, F, IP, P, WB
0.5 ml / €500.00
  Acris Antibodies GmbH

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