Stem Cell Markers: Antibodies to Primordial Germ Cells and Ectoderm - FocusOn 104

Acris Antibodies offers an extensive range of antibodies for stem cells. In this FocusOn we present markers to primordial germ cells and ectoderm (see “Product list”).

Primordial germ cell markers

Dead-box protein 4 (DDX4) is a homolog of the Drosophila protein VASA. DDX4 is a germline marker expressed only in the ovary and testis. DDX4 is expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage and in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Nanos2 (Nos2) is required to support proliferation and self-renewal of proximal germ cells in males only. Nanos2 probably regulates translation of specific mRNAs by associating with the 3‘-UTR of mRNA targets, and it is essential for spermatogonia formation. The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.

Fig. 1: Formalin-fixed and paraffin-embedded (FFPE) human testis tissue reacted with DDX4 antibody (N-term) Cat.-No. AP11351PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 1: Formalin-fixed and paraffin-embedded (FFPE) human testis tissue reacted with DDX4 antibody (N-term) Cat.-No. AP11351PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 2: Human testis tissue (FFPE) reacted with Nanos2 antibody Cat.-No. AP12355PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 2: Human testis tissue (FFPE) reacted with Nanos2 antibody Cat.-No. AP12355PU-N; peroxidase-conjugated secondary antibody, followed by DAB staining
Fig. 3: Western blot analysis of Nanos2 in human testis lyate with Cat.-No. AP30581PU-N at (A) 2 and (B) 4 µg/ml
Fig. 3: Western blot analysis of Nanos2 in human testis lyate with Cat.-No. AP30581PU-N at (A) 2 and (B) 4 µg/ml

Ectodermal markers

GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The most common form affects infants and young children. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity and a more slowly progressive course.

Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord. Olig2 is also a marker for the most common primary tumors of the human brain thought to be of glial cell origin. Therefore, there is diagnostic potential of Olig markers to augment identification of oligodendroglial tumors.

Fig. 4: Immunohistochemistry staining of human cerebellum (FFPE sections) with GFAP antibody clone GF-01 Cat.-No. BM4069S
Fig. 4: Immunohistochemistry staining of human cerebellum (FFPE sections) with GFAP antibody clone GF-01 Cat.-No. BM4069S
Fig.5: Immunohistochemical staining: CCF STTG1 cell line (fixed with 5% acetic acid methanol solution) stained with GFAP-Cy3 antibody Cat.-No. AM08204C3-N; Cells were then fixed and the slide was mounted with DAPI
Fig.5: Immunohistochemical staining: CCF STTG1 cell line (fixed with 5% acetic acid methanol solution) stained with GFAP-Cy3 antibody Cat.-No. AM08204C3-N; Cells were then fixed and the slide was mounted with DAPI
Fig. 6: Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate:Lane 1: immunostaining with anti GFAP (clone GF-02) Cat.-No. AM03085PU-N;Lane 2: immunostaining with mouse IgM isotype control (clone PFR-03) Cat.-No. AM03111PU-N
Fig. 6: Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate:
Lane 1: immunostaining with anti GFAP (clone GF-02) Cat.-No. AM03085PU-N;
Lane 2: immunostaining with mouse IgM isotype control (clone PFR-03) Cat.-No. AM03111PU-N
Fig. 7: Immunoperoxidase staining of monoclonal antibody to Olig2 Cat.-No. H00010215-M03 on human placenta (FFPE)
Fig. 7: Immunoperoxidase staining of monoclonal antibody to Olig2 Cat.-No. H00010215-M03 on human placenta (FFPE)

Further readings

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DDX4 (C-term) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term) (Cat.#AP11352PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GFAP (C-term) antibody

Human Eye stained with GFAP antibody Cat.-No AM09113PU Rabbit IgG SP78 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

GFAP (C-term) antibody

Human Eye stained with GFAP antibody Cat.-No AM09113PU Rabbit IgG SP78 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

GFAP antibody

Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: immunostaining with anti-GFAP (clone GF-02; AM03085PU-N). Lane 2: immunostaining with Isotype mouse IgM control (clone PFR-03; AM03111PU-N) Mouse IgM GF-02 Purified Hu, Por C, ICC/IF, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

GFAP antibody

GFAP Mouse IgG2b SB61b Biotin Hu C, WB
0.5 mg / €310.00
  Acris Antibodies GmbH

GFAP antibody

GFAP Mouse IgG2b SB61b HRP Hu C, WB
1 ml / €310.00
  Acris Antibodies GmbH

GFAP antibody

GFAP Mouse IgG2b SB61b Purified Hu C, WB
0.5 mg / €300.00
  Acris Antibodies GmbH

GFAP antibody

GFAP Mouse IgG1 GF-01 Purified Fe, Hu, Por C, P, WB
0.2 mg / €350.00
  Acris Antibodies GmbH

GFAP antibody

Immunohistochemistry staining of human brain cortex (paraffin-embedded sections) with anti-GFAP antibody (clone GF-01). Mouse IgG2b GF-01 Purified Fe, Hu, Por ICC/IF, IP, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

NANOS2 (C-term) antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOS2 antibody (C-term)(Cat.#AP12355PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

NANOS2 Control Peptide

NANOS2
50 µg / €160.00
  Acris Antibodies GmbH

NANOS2 Control Peptide

NANOS2
50 µg / €160.00
  Acris Antibodies GmbH

OLIG2 antibody

Immunoperoxidase of monoclonal antibody to OLIG2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 3C9 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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