Antibodies to TRAIL Protein Family - FocusOn 022

The TNF-related apoptosis-inducing ligand (TRAIL, TNFSF10) is a member of the TNF superfamily. The interaction of TRAIL with its receptors (see below) either induces or blocks apoptosis.

The TRAIL ligands TRAIL-R1 (DR4, CD261), TRAIL-R2 (Apo2, DR5, CD262, TRICK2) and TRAMP (DR3, Apo-3, WSL-1, LARD, DDR3, TR3) activate NF-kappaB, and induce TRAIL-mediated apoptosis. Initially, TRAMP (gene name: TNFRSF25) was thought to be a receptor for TWEAK (see Focus on No. 18 - Antibodies to TWEAK and TWEAK Receptor), but further studies have shown that TWEAK could induce apoptosis via receptors distinct from TRAMP.

TRAIL-R3 (DcR1, CD263, TRID, LIT) is a glycophospholipid-anchored cell surface protein. It has an extracellular TRAIL binding domain and lacks the cytoplasmic death domain (DD) shared by several members of the TNF receptor superfamily. TRAIL-R4 (DcR2, CD264, TRUNDD) is a transmembrane cell surface protein. It has an extracellular TRAIL binding domain and a truncation in the DD of its cytoplasmic tail. TRAIL-R3 (gene name: TNFRSF10C) and TRAIL-R4 (gene name: TNFRSF10D) protect cells from TRAIL-mediated cytotoxicity: They bind to TRAIL and inhibit binding of TRAIL to TRAIL-R1 (gene name: TNFRSF10A and TRAIL-R2 (gene name: TNFRSF10B).

Antibody Tools for the Detection of TRAIL and TRAIL receptors

Acris Antibodies offers a full panel of antibodies to members of the TRAIL family. These antibodies are available in several different formats and package sizes.

AP09025SU-N CD253 antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 µg/ml.
Fig.1: Cat.-No. AP09025SU-N CD253 antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 µg/ml.
Immunofluorescence (Confocal Microscopy): SM3131A CD261 antibody staining of HeLa Human Cervix Carcinoma cell line transfected with TRAIL-R1 expression plasmid.
Fig.2: Immunofluorescence (Confocal Microscopy): Cat.-No. SM3131A CD261 antibody staining of HeLa Human Cervix Carcinoma cell line transfected with TRAIL-R1 expression plasmid.
Western blot analysis of 20 µg of total cell lysate from HeLa cells with anti-DR4 antibody SM7030P at 2 µg/ml dilution.
Fig.3: Western blot analysis of 20 µg of total cell lysate from HeLa cells with anti-DR4 antibody Cat.-No. SM7030P at 2 µg/ml dilution.
Western blot analysis of 20 µg of whole cell lysates from HL60 cells with anti-DR5 antibody at 5 µg/ml dilution.
Fig.4: Western blot analysis of 20 µg of whole cell lysates from HL60 cells with anti-DR5 antibody at 5 µg/ml dilution.

Available Antibody Panel to the TRAIL Protein Family

TRAIL antibody (TNFSF10 antibody, CD253 antibody), TRAIL-R1 / TRAILR1 (TNFRSF10A , DR4 , CD261 ), TRAIL-R2 / TRAILR2 (TNFRSF10B , DR5 , CD262 ), TRAIL-R3 / TRAILR3 (TNFRSF10C , DcR1 , CD263 ), TRAIL-R4 / TRAILR4 (TNFRSF10D , DcR2 , CD264 ), TRAMP (TNFRSF25 , DR3 , APO3 , WSL- 1, LARD ).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD253 / TRAIL antibody

CD253 / TRAIL Mouse IgG1 B-S23 Azide Free Hu F, FN
0.2 mg / €270.00
  Acris Antibodies GmbH

CD253 / TRAIL antibody

A typical staining pattern with the B-S23 monoclonal antibody of IFNa2+CD3 mAb+CD28 mAb activated lymphocytes Mouse IgG1 B-S23 Biotin Hu F
1ml (100 Tests) / €270.00
  Acris Antibodies GmbH

CD253 / TRAIL antibody

CD253 / TRAIL Mouse IgG1 B-S23 Purified Hu F
2ml (200 Tests) / €250.00
  Acris Antibodies GmbH

CD253 / TRAIL antibody

A typical staining pattern with the B-S23 monoclonal antibody of IFNa2+CD3 mAb+CD28 mAb activated lymphocytes Mouse IgG1 B-S23 PE Hu F
1ml (100 Tests) / €290.00
  Acris Antibodies GmbH

CD253 / TRAIL Control Peptide

CD253 / TRAIL
50 µg / €160.00
  Acris Antibodies GmbH

CD261 / TRAILR1 antibody

Figure 2. Staining DR4 in Kidney tissue by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD261 / TRAILR1 Control Peptide

CD261 / TRAILR1
50 µg / €160.00
  Acris Antibodies GmbH

CD261 / TRAILR1 antibody

CD261 / TRAILR1 Mouse IgG1 DR-4-02 FITC Hu F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD261 / TRAILR1 antibody

Immunofluorescence staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02). Mouse IgG1 DR-4-02 Endotoxin Low Hu
0.1 mg / €260.00
  Acris Antibodies GmbH

CD261 / TRAILR1 antibody

Staining of HeLa cells with Mouse Anti Human  CD261 antibody (SM3131P). Mouse IgG1 DR-4-02 Purified Hu F, IP
0.2 mg / €430.00
  Acris Antibodies GmbH

CD261 / TRAILR1 antibody

CD261 / TRAILR1 Mouse IgG1 DR-4-02 PE Hu F
0.1 mg / €410.00
  Acris Antibodies GmbH

CD262 / TRAILR2 antibody

CD262 / TRAILR2 Mouse IgG1 DR5-01-1 FITC Hu F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD262 / TRAILR2 antibody

CD262 / TRAILR2 Mouse IgG1 DR5-01-1 Aff - Purified Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD262 / TRAILR2 antibody

CD262 / TRAILR2 Mouse IgG1 DR5-01-1 PE Hu F
0.1 mg / €390.00
  Acris Antibodies GmbH

CD262 / TRAILR2 (C-term) antibody

Formalin-Fixed Paraffin-Embedded Human Prostate stained with DR5 antibody AP07306PU-N at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-streptavidin and Chromogen. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD262 / TRAILR2 (isoform 2) (388-407) antibody

AP08473PU-N CD262 antibody staining of Formalin-Fixed Paraffin-Embedded Human Breast at 10 µg/ml. Rabbit IgG Aff - Purified Hu F, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD262 / TRAILR2 (380-398) antibody

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Prim P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD263 / TRAILR3 antibody

CD263 / TRAILR3 Mouse IgG1 2B8 FITC Hu F, IP
0.1 mg / €400.00
  Acris Antibodies GmbH

CD263 / TRAILR3 antibody

CD263 / TRAILR3 Mouse IgG1 2B8 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD263 / TRAILR3 antibody

CD263 / TRAILR3 Mouse IgG1 TRAIL-R3-02 Azide Free Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

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