Antibodies to Vascular Endothelial Growth Factor Receptor (VEGFR) Family: VEGFR-1 antibody, VEGFR-2 antibody and VEGFR-3 antibody - FocusOn 005

Within the last ten years strong evidence has emerged for the essential role of Vascular Endothelial Growth Factor (VEGF) in vasculogenesis and angiogenesis. The development of blood vessels includes two processes. Vasculogenesis is the embryologic formation of blood islands, the earliest vascular system, by the differentiation from mesoderm of angioblasts and hemopoietic precursor cells. Angiogenesis is the sprouting of pre-existing vessels to form the vascular tree. In addition to a key role in embryological development, angiogenesis is essential for such things as wound healing and tumor growth. While many growth factors exhibit angiogenic activity (FGFs, PDGF, TGF-α and HGF), evidence points to a special role for VEGF. VEGF is a dimeric glycoprotein that stimulates endothelial cells, induces angiogenesis and increases vascular permeability.

VEFG Receptor (VEGFR)

The receptors for VEGF are VEGFR-1 (Flt-1) and VEGFR-2 (KDR in humans and Flk-1 in mice). Homozygous mutants of the VEGF receptors lead to lack of vasculogenesis and death of mouse embryos at about day 8, indicating that VEGF receptors are essential for formation of a normal vasculature. In addition to VEGF (VEGF-A) there exist three additional VEGF family members: VEGF-B, VEGF-C and VEGF-D. Less is known about the function and regulation of VEGF-B, -C, and -D, but they do not seem to be regulated by the major pathways that control VEGF-A. VEGF-C and D are ligands for VEGFR-2 but also for the third VEGF Receptor VEGFR-3 (Flt-4), whereas VEGF-B binds only to VEGFR-1.

Like other growth factor receptors, upon ligand binding VEGFR-2 dimerizes and is autophosphorylated on multiple tyrosine residues. These phosphotyrosine sites are involved in regulation of intrinsic tyrosine kinase activity or serve as binding sites for SH2 domains and phosphotyrosine binding (PTB) domains of downstream signaling proteins.

Fig. 1: Formalin fixed paraffin embedded (FFPE) human angiosarcoma stained with VEGFR-1/Flt-1 antibody Cat.-No. DP077-05
Fig. 1: Formalin fixed paraffin embedded (FFPE) human angiosarcoma stained with VEGFR-1/Flt-1 antibody Cat.-No. DP077-05
Fig. 2: Immunofluorescence staining (green) using a polyclonal antibody directed against the C-terminal end of native soluble VEGFR-1/Flt-1 Cat.-No. DP3522 (2a) and a monoclonal antibody directed against the extracellular domain of the membrane-bound VEGFR-1/Flt-1 Cat.-No. DM3507 (2b). Two neighboring sections of a Human Vein (V), located near a hemangioma, are visualized. The antibody against the soluble VEGFR-1/Flt-1 marked single cells (arrows) within the media and adventitia of the vein. The antibody against the membrane-bound VEGFR-1/Flt-1 marked single cells (arrows) and the endothelium (arrowhead) of the vein. Cell nuclei are stained with Dapi (blue).The experiment was performed by K. Butler and J. Wilting, University Göttingen, Germany
Fig. 2: Immunofluorescence staining (green) using a polyclonal antibody directed against the C-terminal end of native soluble VEGFR-1/Flt-1 Cat.-No. DP3522 (2a) and a monoclonal antibody directed against the extracellular domain of the membrane-bound VEGFR-1/Flt-1 Cat.-No. DM3507 (2b). Two neighboring sections of a Human Vein (V), located near a hemangioma, are visualized. The antibody against the soluble VEGFR-1/Flt-1 marked single cells (arrows) within the media and adventitia of the vein. The antibody against the membrane-bound VEGFR-1/Flt-1 marked single cells (arrows) and the endothelium (arrowhead) of the vein. Cell nuclei are stained with Dapi (blue).
The experiment was performed by K. Butler and J. Wilting, University Göttingen, Germany
Fig. 3: Human placenta (FFPE) stained with anti-VEGFR-2/Flk-1 antibody Cat.-No. AP15556PU-N
Fig. 3: Human placenta (FFPE) stained with anti-VEGFR-2/Flk-1 antibody Cat.-No. AP15556PU-N
Fig. 4: FACS analysis with primary HUVECs using anti-human VEGFR-2 Clone 4H3 Cat.-No. DM3522P
Fig. 4: FACS analysis with primary HUVECs using anti-human VEGFR-2 Clone 4H3 Cat.-No. DM3522P
Fig. 5: Formalin fixed paraffin embedded (FFPE) human angiosarcoma stained with VEGFR-2/Flk-1 antibody Cat.-No. DP076-05
Fig. 5: Formalin fixed paraffin embedded (FFPE) human angiosarcoma stained with VEGFR-2/Flk-1 antibody Cat.-No. DP076-05
Fig. 6: Human placenta (FFPE) stained with anti-VEGFR-3/Flt-4 antibody Cat.-No. AP15558PU-N
Fig. 6: Human placenta (FFPE) stained with anti-VEGFR-3/Flt-4 antibody Cat.-No. AP15558PU-N

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD309 / VEGFR-2 / Flk-1 (N-term) antibody

Human Prostate Adenocarcinoma stained with FLK1 Antibody Cat.-No AM21042PU (Clone SP123) Rabbit IgG SP123 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (N-term) antibody

Human Prostate Adenocarcinoma stained with FLK1 Antibody Cat.-No AM21042PU (Clone SP123) Rabbit IgG SP123 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Western Analysis of anti-Human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Mouse IgG1 KDREIC Purified Hu C, E, F, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Figure 5. Consecutive sections of unfixed, human foreskin. A) Staining with anti-soluble VEGFR2/KDR antibodies (Cat.-No AP26034PU-L). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR (Cat.-No DM3522P). Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium. Provided by Prof. J. Wilting, Göttingen, Germany. Mouse IgG1 4H3 Purified Hu E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Up-regulation of VEGFR-2 in vein ECs of an intact Human umbilical cord by bFGF: A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2 antibody Cat.-No DM3523P as detection antibody. (Bernhard Barleon et al., unpublished data!) Mouse IgG1 4 (20I6) Purified Hu C, E, F, ICC/IF, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.2 mg / €370.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Immunohistochemistry with Cryo sections using polyclonal anti-Human VEGFR-2/KDR antibody (RT, Cat.-No DP3509S). The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology Robert-Koch-Str. 8, D-35037 Marburg, Germany. Rabbit Azide Free Hu C, E, FN, IP, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Human placenta stained with Anti-Flk-1 antibody Rabbit Purified Hu, Ms P
1 ml / €440.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Human placenta stained with Anti-Flk-1 antibody Rabbit Purified Hu, Ms P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using VEGFR-2/KDR mouse mAb Cat.-No. AM06371SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 4B4 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (esKDR) antibody

Immunofluorescence staining with consecutive sections of unfixed, human foreskin. A) Staining with anti-sVEGFR2/KDR antibodies (Cat.-No AP26034PU-N). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR. Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium.
Provided by Prof. J. Wilting, Göttingen, Germany. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (esFlk1) antibody

Western blot was performed using monoclonal anti-Mouse esFlk-1 (Cat.-No DM3522P) recognizing the soluble as well as the transmembrane form of Flk-1 and poyclonal antibody (Cat.-No AP26035PU) directed against the unique C-terminal end of the endogenous esFlk-1 (GMEASLGDRIAMP) recognizing solely the endogenous form. There is no cross reactivity of both antibodies with human esKDR. The endogenous esFlk-1 generated by alternative splicing consists of the first 6 Ig-like loops. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €270.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

FACS analysis of VEGFR-2/KDR expression in HUVE cells. Mouse IgG1 KDREIC Biotin Hu C, E, F, WB
50 µg / €350.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

Figure 1. FACS analysis with primary human microvascular endothelial cells (HMVEC) using biotinylated monoclonal antibody DM3522B. Mouse IgG1 4H3 Biotin Hu E, F, WB
50 µg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

CD309 / VEGFR-2 / Flk-1 Mouse IgG1 4 (20I6) Biotin Hu C, E, F, ICC/IF, WB
50 µg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 (C-term) antibody

Formalin fixed paraffin embedded human angiosarcoma stained with Flk-1 antibody Rabbit Purified Hu, Ms, Rt P
0.5 ml / €450.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

CD309 / VEGFR-2 / Flk-1 Rabbit Biotin Hu E, FN, IP, WB
50 µg / €330.00
  Acris Antibodies GmbH

CD309 / VEGFR-2 / Flk-1 antibody

CD309 / VEGFR-2 / Flk-1 Rabbit Aff - Purified Hu E, FN, IP, WB
50 µg / €270.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 antibody

Standard ELISA assay with coating of 2.5 µg/ml Mouse sFlt-1-Fc using anti Human Flt-1 antibodies Mouse IgG1 FLTEWC Purified Hu, Ms E, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (C-term) antibody

Figure 1. AP15557PU-N VEGFR-1/Flt-1 antibody staining of Human Placenta. Rabbit Aff - Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

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