Product Description for Cell Fractionation Kit
Properties for Cell Fractionation Kit
|Synonyms||Mitochondria/Cytosol Fractionation Kit|
|Reactivity||Bov, Hu, Ms, Rt|
|PDF datasheet||View Datasheet|
|Add. information||Cell Fractionation Kit Components:
350mL of Buffer A (2 x 175 mL aliquots)
25 µL of Detergent I
1 mL of Detergent II
10 mL of 5X SDS Sample Buffer.
|Application||Preparation of cytosolic.
Mitochondrial and nuclear fractions in microplate format.
|Background||The Cell Fractionation Kit allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Unlike competing products, this Cell Fractionation Kit does not require mechanical disruption of the sample. This is an important performance characteristic, as mechanical disruption can also disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. Particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.
A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (MS862)
|General Readings||Liu et al., 2009. Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.|
|Storage||Store Buffer A, Detergent HT II, Detergent HT III and 5X SDS Sample Buffer at -20°C. Store Detergent HT I at -80°C. Store the collection plates and seal films at room temperature.|