NB900-79773 AEC Chromogen/Substrate Kit

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3 vials / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

NB900-79773

Product Description for AEC Chromogen/Substrate Kit

Product is tested for Frozen Sections, Paraffin Sections.

Properties for AEC Chromogen/Substrate Kit

Product Category Detection Kits & Signal Boosters
Quantity 3 vials
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Solution Preparation
1. Add 5 ml of distilled or de-ionized water to a test tube.
2. Add two drops of concentrated buffer
3. Add two drops of concentrated AEC chromogen and mix.
4. Add two drops of 3% H2 O2 substrate solution and mix.
* Working chromogen solution is stable for 6 hr. Any solution not used after this period should be discarded.

Staining
1. Once tissue sections have been incubated with peroxidase, wash them with buffer thoroughly.
2. Wipe the glass to remove excess of buffer and add enough of the AEC working solution to cover the tissue sections.
3. Incubate for 10-20 minutes at room temperature. For best results, observe signal development under the microscope. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in wash buffer.
Application , Solution Preparation 1. Add 5 ml of distilled or de-ionized water to a test tube. 2. Add two drops of concentrated buffer 3. Add two drops of concentrated AEC chromogen and mix. 4. Add two drops of 3% H2 O2 substrate solution and mix. * Working chromogen solution is stable for 6 hr. Any solution not used after this period should be discarded. Staining 1. Once tissue sections have been incubated with peroxidase, wash them with buffer thoroughly. 2. Wipe the glass to remove excess of buffer and add enough of the AEC working solution to cover the tissue sections. 3. Incubate for 10-20 minutes at room temperature. For best results, observe signal development under the microscope. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in wash buffer.
Background AEC is a chromogen of choice when performing immunoperoxidase staining. It represents a popular alternative to DAB due to its lower toxicity. AEC produces a red precipitate at positive sites and provides excellent contrast with blue hematoxylin counter stains. Note that specimens stained in AEC can not be dehydrated in ethanol and should be mounted in aqueous based mounting medium.
Storage Store at 4C. Do not freeze.
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