NB900-78646 BCIP/NBT Alkaline Phosphatase Substrate

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15 ml / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

NB900-78646

Properties for BCIP/NBT Alkaline Phosphatase Substrate

Product Category Detection Kits & Signal Boosters
Quantity 15 ml
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information 1.) Once tissue sections have been incubated with alkaline phosphatase, wash them with buffer thoroughly.
2.) Wipe the glass to remove excess of buffer and add enough drops of the BCIP/NBT solution to cover the tissue sections.
3.) Incubate for 10-30 minutes at room temperature. For the best results, look under the microscope for the signal development. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in the wash buffer.
Application immunohistochemistry, Western Blot, 1.) Once tissue sections have been incubated with alkaline phosphatase, wash them with buffer thoroughly. 2.) Wipe the glass to remove excess of buffer and add enough drops of the BCIP/NBT solution to cover the tissue sections. 3.) Incubate for 10-30 minutes at room temperature. For the best results, look under the microscope for the signal development. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in the wash buffer. , immunohistochemistry, Western Blot
Background Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the use of an insoluble colored substrate. Novus' BCIP/NBT is a stable ready to use solution which produces a blue colored end product at the positive sites and provides excellent contrast with red nuclear fast counter stain. Specimens stained in BCIP/NBT can not be dehydrated in ethanol and should be mounted in aqueous mounting medium Alkaline phosphatase cleaves the phosphate group from BCIP to produce precipitating indoxyl group. This group then dimerizes resulting in the reduction of the NBT to produce a dark blue insoluble Formazan product.
Storage Store at 4C. Do not freeze.
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