Properties for ECL Kit (Mouse IgM) for WB
|Product Category||Detection Kits & Signal Boosters|
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer certificates||ISO 9001|
|Add. information||Kit components:
1. Blocking buffer: 10g protein dry powder (skimmed milk powder).
2. HRP‐conjugated goat anti‐mouse IgM: Affinity purified antibody. 2000‐10000×.
3. Enhanced chemiluminescent chromogenic reagent, containing:
-Chromogenic reagent A: 5ml. 20×.
-Chromogenic reagent B: 5ml. 20×.
Material Required But Not Provided:
Nitrocellulose or PVDF membrane.
-Diluent Buffer (for preparation of blocking reagent and antibody dilutions): Add 2.42g Tris, 9g NaCl, 850‐900μl pure acetic acid into 1000ml distilled water, adjust pH to 7.2‐7.6.
-Wash Buffer: Add 0.5 ml of TWEEN 20 into 1000 ml of diluent buffer.
-Primary antibody: User provided. This kit applies to the primary IgM antibodies raised from mouse.
|Application||• “Mouse IgM” refers to the origin of animal species of the primary antibody, not the origin of thespecimen. This kit applies to the primary antibodies raised from mouse.• Every kit has sufficient reagents for 800 cm2 of membranes.|
|Storage||4°C for 1 year.|