BB-SA1082 FITC + POD Rabbit IgG SABC Kit

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Properties for FITC + POD Rabbit IgG SABC Kit

Product Category Detection Kits & Signal Boosters
Quantity 1 kit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Add. information Kit components:
1. Normal goat serum blocking reagent: 10×, 5 ml, for blocking tissue sections.
2. Biotinylated Secondary Antibody (Goat Anti-rabbit IgG): 100×, 0.5ml (2mg/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2).
3. SABC-FITC+Peroxidase (FITC and peroxidase conjugated streptavidin): 100×, 0.5ml (2mg/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals.
4. Three drop bottles (For dilution use).
Material Required But Not Provided:
2. 0.02M PBS (pH 7.2~7.6): 8.5g sodium chloride, 2.8g anhydrous Na2HPO4 and 0.4g anhydrous NaH2PO4 in 1000ml of distilled water. (The weight should be adjusted accordingly if hydrous phosphates are used.)
3. 0.01 M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7•2H2O) and 0.4g citric acid monohydrate (C6H8O7•H2O) in 1000ml of distilled water.
4. DAB Chromogenic Kit (Catalog number: BB-AR1022 or BB-AR1025), to be used with SABC-POD.
5. 0.1% trypsinase or the compound digest solution (Catalog number: BB-AR0022).
Application IHC-P, IHC-F, IC
IHC(P): 1:50-200IHC(F): 1:50-200IC: 1:50-200Optimal dilutions should be determined by end users.
Rabbit IgG refers to the animal origin of the primary antibody, not the origin of the specimen. This kit must be used on primary antibodies from rabbit.
Storage 4°C for one year. Avoid freezing
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