Properties for HRP Goat IgG SABC Kit
| Product Category | Detection Kits & Signal Boosters |
|---|---|
| Quantity | 1 kit |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Boster Immunoleader |
| Manufacturer certificates | ISO 9001 |
Datasheet Extract
| Add. information | Kit components: 1. Normal rabbit serum blocking reagent: 10×, 10ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Rabbit Anti-goat IgG): 100~200×, 1ml (2mg/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-POD (Peroxidase conjugated streptavidin): 100~200×, 1ml (2mg/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals. 4. Three drop bottles (For dilution use). Material Required But Not Provided: 1. APES or POLY-L-LYSINE. 2. 0.02M PBS (pH 7.2~7.6): 8.5g sodium chloride, 2.8g anhydrous Na2HPO4 and 0.4g anhydrous NaH2PO4 in 1000ml of distilled water. (The weight should be adjusted accordingly if hydrous phosphates are used.) 3. 0.01 M Citrate Buffer: 3g sodium citrate dihydrate (C6H5Na3O7•2H2O) and 0.4g citric acid monohydrate (C6H8O7•H2O) in 1000ml of distilled water. 4. DAB Chromogenic Kit (Catalog number: BB-AR1022 or BB-AR1025). 5. 0.1% trypsinase or the compound digest solution (Catalog number: BB-AR0022). |
|---|---|
| Application | IHC-P, IHC-F, IC IHC(P): 1:50-200IHC(F): 1:50-200IC: 1:50-200Optimal dilutions should be determined by end users. Goat IgG refers to the animal origin of the primary antibody, not the origin of the specimen. This kit must be used on primary antibodies from goat. |
| Storage | 4°C for one year. Avoid freezing |
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