EA101028 AFP ELISA Kit

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1 kit / €245.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

EA101028

Properties for AFP ELISA Kit

Product Category ELISA Kits
Quantity 1 kit
Synonyms AFP
Reactivity Hu
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Add. information
Interference:
No significant interference observed with available related molecules.
Application
Specificity:
Natural and recombinant human AFP
Assay Type:
Solid Phase Sandwich ELISA
Signal:
Colorimetric
Sample Type:
Cell culture supernatant, serum, plasma
Sample Volume:
25 ul
Curve Range:
0 - 160 IU/mL
Background The AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of specimen sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti-AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of AFP in the specimen sample.
Assaytime 3 hours
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