601-0121 Colorimetric ATPase Assay Kit

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Properties for Colorimetric ATPase Assay Kit

Product Category ELISA Kits
Quantity 480 Tests
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

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The ATPase colorimetric assay kit employs a 96-well plate format with all reagents necessary for measuring ATPase activity. The kit contains specially purified Pi -free ATP to ensure the lowest possible background signals. It also contains PiColorLock Gold reagent (an improved malachite green formulation) with additives to prevent background signals arising out of nonenzymatic ATP hydrolysis. Assays can be read anywhere in the wavelength range 590-660nm.


2.1 Components in the 2-plate ATP assay kit (5-plate kit amounts in brackets)

Store at 4C:

1 x 10ml of PiColorLock Gold* (1 x 25ml)
1 x 0.25ml of Accelerator (1 x 0.5ml)
1 x 5ml of Stabiliser (1 x 10ml)
1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)
1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)
1 x 5ml of 0.1mM Pi standard (1 x 10ml)

Store at -70C:

2 x 1ml specially purified ATP (5 x 1ml)

Store at room temperature:

2 x 96-well plates (5 plates)

*Exercise caution


This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.

Storage Storage is content dependent.
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