602-0121 Colorimetric GTPase Assay Kit

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Properties for Colorimetric GTPase Assay Kit

Product Category ELISA Kits
Quantity 480 Tests
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

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2.1 Components in the 2-plate GTP assay kit:

(5-plate kit amounts in brackets)

Store at 4C:

1 x 10ml of PiColorLock Gold* (1 x 25ml)
1 x 0.25ml of Accelerator (1 x 0.5ml)
1 x 5ml of Stabiliser (1 x 10ml)
1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)
1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)
1 x 5ml of 0.1mM Pi standard (1 x 10ml)

Store at -70C:

2 x 1ml specially purified GTP (5 x 1ml)

Store at room temperature:

2 x 96-well plates (5 plates)

*Exercise caution


This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.

Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.

Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed

Storage Storage is content dependent.
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