Properties for DNA Damage (8-OHdG) ELISA kit
|Product Category||ELISA Kits|
|Shipping to||Europe only|
|PDF datasheet||View Datasheet|
|Background||8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. 1-4 Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.
In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7- 13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/ml as determined by LC-MS.
This is a competitive assay that can be used for the quantification of 8HdG in urine, cell culture, plasma and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxy Guanosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 0.94 - 60 ng/ml, with a sensitivity of 0.59 ng/ml and a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol. It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.
|Caution||WARNING: This kit is not for human or animal disease diagnosis or therapeutic drug use.
|Storage||All reagents are stable as supplied at 4°C, except the standard, which should be stored at -20°C. For optimum storage, the 8-OHdG Standard should be aliquotted into smaller portions and then stored appropriately. Avoid repeated freeze/thaw cycles (10 μl of Standard can prepare a triplicate standard curve).|
|Range||0.94 - 60 ng/ml|