Properties for Human EGF ELISA Kit
|Product Category||ELISA Kits|
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer certificates||ISO 9001|
|Add. information||Sensitivity < 1 pg/ml|
|Application||Sandwich ELISA kit of Quantitative Detection for Human EGF
This kit is used for quantitative detection of human EGF in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine.
There is no detectable cross-reactivity with other relevant proteins.
|Background||Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds.1 EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival.2 It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.3 EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.4|
|Storage||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|