BB-EK0886 Human Endostatin ELISA Kit

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Properties for Human Endostatin ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Hu
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Swiss Prot Num:
Add. information Sensitivity < 10 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Human Endostatin
This kit is used for quantitative detection of human Endostatin in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
There is no detectable cross-reactivity with other relevant proteins.
Background Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar toangiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
General Readings
  1. Felbor U, Dreier L, Bryant RA, Ploegh HL, Olsen BR, Mothes W. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 2000 Mar 15;19(6):1187-94. PubMed PMID: 10716919. (Free PMC Article available, 7 images available)
  2. Oh SP, Warman ML, Seldin MF, Cheng SD, Knoll JH, Timmons S, et al. Cloning of cDNA and genomic DNA encoding human type XVIII collagen and localization of the alpha 1(XVIII) collagen gene to mouse chromosome 10 and human chromosome 21. Genomics. 1994 Feb;19(3):494-9. PubMed PMID: 8188291.
  3. Shi H, Huang Y, Zhou H, Song X, Yuan S, Fu Y, et al. Nucleolin is a receptor that mediates antiangiogenic and antitumor activity of endostatin. Blood. 2007 Oct 15;110(8):2899-906. Epub 2007 Jul 5. PubMed PMID: 17615292.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 156-10,000 pg/ml
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