Properties for Human Fractalkine/CX3CL1 ELISA Kit
|Product Category||ELISA Kits|
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer certificates||ISO 9001|
|Add. information||Sensitivity < 50 pg/ml|
|Application||Sandwich ELISA kit of Quantitative Detection for Human Fractalkine/CX3CL1
This kit is used for quantitative detection of human Fractalkine in cell culture supernates, serum and plasma(heparin, EDTA), saliva, urine and human milk.
There is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC < 0.2%.
|Background||Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5|
|Storage||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|