BB-EK0421 Human IL-12(p70) ELISA Kit

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96 Tests / €340.00
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BB-EK0421

Properties for Human IL-12(p70) ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Hu
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Immunogen
Swiss Prot Num:
O60595
Add. information Sensitivity < 2 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Human IL-12(p70)
This kit is used for quantitative detection of human IL-12(p70) in cell culture supernates and serum.
There is no detectable cross-reactivity with other relevant proteins.
Background Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.
General Readings
  1. Toubai T, Tanaka J, Ota S, Fukuhara T, Hashino S, Kondo T, et al. Effect of granulocyte colony-stimulating factor on IL-12 p40 production during chemotherapy for B-cell lineage non-Hodgkin's lymphoma patients. Eur J Haematol. 2006 Nov;77(5):403-9. Epub 2006 Aug 23. PubMed PMID: 16930137.
  2. Nakamura T, Kimura H, Kato M, Kurashige S, Wakamatsu K. A sensitive and reliable quantification method for mouse interleukin-12 p70 based on fluorometric sandwich ELISA (FS-ELISA). Cell Biol Int. 2007 Feb;31(2):173-9. Epub 2006 Oct 28. PubMed PMID: 17145187.
  3. Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, et al. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction. Infect Immun. 2006 May;74(5):2831-8. PubMed PMID: 16622221. (Free PMC Article available, 5 images available)
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 7.8-500 pg/ml
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