BB-EK0437 Human Leptin ELISA Kit

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Properties for Human Leptin ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Hu
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Swiss Prot Num:
Add. information Sensitivity < 10 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Human Leptin
This kit is used for quantitative detection of human Leptin in cell culture supernates, serum and plasma( heparin, EDTA).
There is no detectable cross-reactivity with other relevant proteins.
Background Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.
General Readings
  1. Masuzaki H, Ogawa Y, Sagawa N, Hosoda K, Matsumoto T, Mise H, et al. Nonadipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans. Nat Med. 1997 Sep;3(9):1029-33. PubMed PMID: 9288733.
  2. Masuzaki H, Ogawa Y, Isse N, Satoh N, Okazaki T, Shigemoto M, et al. Human obese gene expression. Adipocyte-specific expression and regional differences in the adipose tissue. Diabetes. 1995 Jul;44(7):855-8. PubMed PMID: 7789654.
  3. Green, E. D.; Maffei, M.; Braden, V. V.; Proenca, R.; DeSilva, U.; Zhang, Y.; Chua, S. C., Jr.; Leibel, R. L.; Weissenbach, J.; Friedman, J. M. The human obese (OB) gene: RNA expression pattern and mapping on the physical, cytogenetic, and genetic maps of chromosome 7. Genome Res. 5: 5-12, 1995.
  4. Auwerx J, Staels B. Leptin. Lancet. 1998 Mar 7;351(9104):737-42. PubMed PMID: 9504534.
  5. Elefteriou F, Takeda S, Ebihara K, Magre J, Patano N, Kim CA, et al. Serum leptin level is a regulator of bone mass. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3258-63. Epub 2004 Feb 20. PubMed PMID: 14978271. (Free PMC Article available, 5 images available)
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 62.5-4000 pg/ml
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