BB-EK0813 Human MIF ELISA Kit

Search for all "Human MIF ELISA Kit"


Properties for Human MIF ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Hu
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Swiss Prot Num:
Add. information Sensitivity < 20 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Human MIF
This kit is used for quantitative detection of human MIF in cell culture supernates, serum, plasma( heparin, EDTA) and human milk.
There is no detectable cross-reactivity with other relevant proteins.
Background Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4. The standard product used in this kit is recombinant human MIF, consisting of 115 amino acids with the molecular mass of 12.5kDa.
General Readings
  1. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7522-6. PubMed PMID: 2552447. (Free PMC Article available)
  2. Kozak CA, Adamson MC, Buckler CE, Segovia L, Paralkar V, Wistow G. Genomic cloning of mouse MIF (macrophage inhibitory factor) and genetic mapping of the human and mouse expressed gene and nine mouse pseudogenes. Genomics. 1995 Jun 10;27(3):405-11. PubMed PMID: 7558020.
  3. Budarf M, McDonald T, Sellinger B, Kozak C, Graham C, Wistow G. Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. Genomics. 1997 Jan 15;39(2):235-6. PubMed PMID: 9027512.
  4. Paralkar V, Wistow G. Cloning the human gene for macrophage migration inhibitory factor (MIF). Genomics. 1994 Jan 1;19(1):48-51. PubMed PMID: 8188240.
  5. Leng L, Bucala R. Insight into the biology of macrophage migration inhibitory factor (MIF) revealed by the cloning of its cell surface receptor. Cell Res. 2006 Feb;16(2):162-8. PubMed PMID: 16474429.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 156-10,000 pg/ml
  • LinkedIn