BB-EK0484 Human PDGF-AB ELISA Kit

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96 Tests / €390.00
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BB-EK0484

Properties for Human PDGF-AB ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Hu
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Immunogen
Swiss Prot Num:
P01127, P04085
Add. information Sensitivity < 2 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Human PDGF-AB
This kit is used for quantitative detection of human PDGF-AB in cell culture supernates, serum and plasma(heparin, EDTA).
There is no detectable cross-reactivity with other relevant proteins.
Background The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.
General Readings
  1. Kelly JD, Raines EW, Ross R, Murray MJ. The B chain of PDGF alone is sufficient for mitogenesis. EMBO J. 1985 Dec 16;4(13A):3399-405. PubMed PMID: 3004957. (Free PMC Article available)
  2. Hermansson M, Nistér M, Betsholtz C, Heldin CH, Westermark B, Funa K. Endothelial cell hyperplasia in human glioblastoma: coexpression of mRNA for platelet-derived growth factor (PDGF) B chain and PDGF receptor suggests autocrine growth stimulation. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7748-52. PubMed PMID: 2845420. (Free PMC Article available)
  3. Collins T, Ginsburg D, Boss JM, Orkin SH, Pober JS. Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis. Nature. 1985 Aug 22-28;316(6030):748-50. PubMed PMID: 4033772.
  4. Stenman G, Rorsman F, Betsholtz C. Sublocalization of the human PDGF A-chain gene to chromosome 7, band q11.23, by in situ hybridization. Exp Cell Res. 1988 Sep;178(1):180-4. PubMed PMID: 3409978.
  5. Dalla-Favera R, Gallo RC, Giallongo A, Croce CM. Chromosomal localization of the human homolog (c-sis) of the simian sarcoma virus onc gene. Science. 1982 Nov 12;218(4573):686-8. PubMed PMID: 6291150.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 31.2-2000 pg/ml
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