Product Description for Influenza A NCP Antigen Capture ELISA - Photometric
Properties for Influenza A NCP Antigen Capture ELISA - Photometric
|Product Category||ELISA Kits|
|Quantity||5 x 96 Tests|
|PDF datasheet||View Datasheet|
|Background||Influenza viruses can be divided into three classes, A, B, and C, largely based upon conserved antigenic differences in the internal nucleoprotein. Influenza A virus, typically encountered more frequently than types B and C, and associated with the majority of serious epidemics, can be further subdivided into strains or subtypes based on antigenic differences in the external hemagglutinin proteins (H1-H16) and neuraminidase proteins (N1-N9).
A variety of wild waterfowl appear to be the predominant natural reservoir for Influenza A viruses and subtypes representing many of the hemagglutinin and neuraminidase combinations can be found circulating in these birds. Historically, human influenza virus infections have been associated with H1N1, H2N2, and H3N2 subtypes of influenza A, although a recent (1997) and significant outbreak in Hong Kong was identified as an H5N1 subtype. This outbreak was not only significant because it resulted in 18 human infections and 6 deaths, but it also represented the first known demonstration of avian influenza virus transmission to humans. Since the 1997 H5N1 outbreak in Hong Kong, additional outbreaks of different influenza A subtypes exhibiting bird to human transmission have been documented; H9N2 in Hong Kong (1999, 2003) and China (1999), H7N2 in The Netherlands (2003), and H5N1 in China (2003) and Southeast Asia (2003-2004). As a result of these outbreaks and other information, there is a significant concern that a human pandemic strain of influenza A could emerge.
The detection limit of the kit varies depending upon the type of sample being evaluated. In assays using recombinant antigen, purified virus, virus in tissue culture fluid or diluted in a "simple" buffer (PBS/BSA), the analytical sensitivity is ~150 pg/ml.