Properties for Lightning Link Conjugation Kit
|Product Category||ELISA Kits|
|Quantity||3 x 100ug Reaction|
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer||Novus Biologicals Inc.|
|Add. information||This conjugation kit contains 3 x 100 ug of PerCP Cy5.5|
|Background||PerCP/Cy5.5 is a tandem conjugate. The PerCP is excited at 482nm and functions as an energy donor for the Cy5.5. Energy is transferred from the PerCP to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PerCP in the form of long wavelength light at 700nm.
The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
|Storage||The kit is shipped at ambient temperature in a tamper-evident polypropylene container. Store at -20C upon receipt.|