Properties for Mouse IL-12(p70) ELISA Kit
|Product Category||ELISA Kits|
|Shipping to||Not USA/Canada|
|PDF datasheet||View Datasheet|
|Manufacturer certificates||ISO 9001|
|Add. information||Sensitivity < 1.5 pg/ml|
|Application||Sandwich ELISA kit of Quantitative Detection for Mouse IL-12(p70)
This kit is used for quantitative detection of mouse IL-12(p70) in cell culture supernates and serum.
There is no detectable cross-reactivity with other relevant proteins.
|Background||Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.|
|Storage||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|