BB-EK0436 Mouse Laminin ELISA Kit

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96 Tests / €350.00
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BB-EK0436

Properties for Mouse Laminin ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Ms
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Immunogen
Swiss Prot Num:
E9PUR4
Add. information Sensitivity < 10 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Mouse Laminin
This kit is used for quantitative detection of mouse Laminin in cell culture supernates, serum and plasma(heparin, EDTA).
There is no detectable cross-reactivity with other relevant proteins.
Background Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation.2 The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids.1 The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.4 The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.5
General Readings
  1. Haaparanta T, Uitto J, Ruoslahti E, Engvall E. Molecular cloning of the cDNA encoding human laminin A chain. Matrix. 1991 Jun;11(3):151-60. PubMed PMID: 1714537.
  2. Sasaki M, Kato S, Kohno K, Martin GR, Yamada Y. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc Natl Acad Sci U S A. 1987 Feb;84(4):935-9. PubMed PMID: 3493487. (Free PMC Article available)
  3. Nagayoshi T, Mattei MG, Passage E, Knowlton R, Chu ML, Uitto J. Human laminin A chain (LAMA) gene: chromosomal mapping to locus 18p11.3. Genomics. 1989 Nov;5(4):932-5. PubMed PMID: 2591971.
  4. Jaye M, Modi WS, Ricca GA, Mudd R, Chiu IM, O'Brien SJ, et al. Isolation of a cDNA clone for the human laminin-B1 chain and its gene localization. Am J Hum Genet. 1987 Oct;41(4):605-15. PubMed PMID: 3661559. (Free PMC Article available)
  5. Sasaki M, Yamada Y. The laminin B2 chain has a multidomain structure homologous to the B1 chain. J Biol Chem. 1987 Dec 15;262(35):17111-7. PubMed PMID: 3680290.
  6. Mattei MG, Weil D, Pribula-Conway D, Bernard MP, Passage E, Van Cong N, et al. cDNA cloning, expression and mapping of human laminin B2 gene to chromosome 1q31. Hum Genet. 1988 Jul;79(3):235-41. PubMed PMID: 3402995.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 156-10,000 pg/ml
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