BB-EK0483 Mouse OPN ELISA Kit

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Properties for Mouse OPN ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Ms
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Swiss Prot Num:
Add. information Sensitivity < 10 pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Mouse OPN
This kit is used for quantitative detection of mouse OPN in cell culture supernates, serum, plasma(heparin, EDTA) and urine.
There is no detectable cross-reactivity with other relevant proteins.
Background Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis.1 Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.
General Readings
  1. Beck GR, Zerler B, Moran E. Phosphate is a specific signal for induction of osteopontin gene expression. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8352-7. PubMed PMID: 10890885. (Free PMC Article available, 7 images available)
  2. Kohri K, Suzuki Y, Yoshida K, Yamamoto K, Amasaki N, Yamate T, et al. Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin. Biochem Biophys Res Commun. 1992 Apr 30;184(2):859-64. PubMed PMID: 1575754.
  3. Fisher LW, McBride OW, Termine JD, Young MF. Human bone sialoprotein. Deduced protein sequence and chromosomal localization. J Biol Chem. 1990 Feb 5;265(4):2347-51. PubMed PMID: 2404984.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 156-10,000 pg/ml
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