BB-EK0500 Mouse SDF-1 ELISA Kit

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Properties for Mouse SDF-1 ELISA Kit

Product Category ELISA Kits
Quantity 96 Tests
Reactivity Ms
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Swiss Prot Num:
Add. information Sensitivity < 10pg/ml
Application Sandwich ELISA kit of Quantitative Detection for Mouse SDF-1
This kit is used for quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin).
There is no detectable cross-reactivity with other relevant proteins.
Background SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1.1 SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene.2 Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes.3,4,5,6 CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes.7 CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide.8 The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.
General Readings
  1. Shirozu M, Nakano T, Inazawa J, Tashiro K, Tada H, Shinohara T, et al. Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene. Genomics. 1995 Aug 10;28(3):495-500. PubMed PMID: 7490086.
  2. De La Luz Sierra M, Yang F, Narazaki M, Salvucci O, Davis D, Yarchoan R, et al. Differential processing of stromal-derived factor-1alpha and stromal-derived factor-1beta explains functional diversity. Blood. 2004 Apr 1;103(7):2452-9. Epub 2003 Oct 2. PubMed PMID: 14525775.
  3. Bleul CC, Fuhlbrigge RC, Casasnovas JM, Aiuti A, Springer TA. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1) J Exp Med. 1996 Sep 1;184(3):1101-9. PubMed PMID: 9064327. (Free PMC Article available)
  4. Ara T, Nakamura Y, Egawa T, Sugiyama T, Abe K, Kishimoto T, et al. Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1). Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5319-23. Epub 2003 Apr 8. PubMed PMID: 12684531. (Free PMC Article available, 3 images available)
  5. Askari AT, Unzek S, Popovic ZB, Goldman CK, Forudi F, Kiedrowski M, et al. Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet. 2003 Aug 30;362(9385):697-703. PubMed PMID: 12957092.
  6. Ma Q, Jones D, Borghesani PR, Segal RA, Nagasawa T, Kishimoto T, et al. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice. Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9448-53. PubMed PMID: 9689100. (Free PMC Article available, 5 images available)
  7. McQuibban GA, Butler GS, Gong JH, Bendall L, Power C, Clark-Lewis I, et al. Matrix metalloproteinase activity inactivates the CXC chemokine stromal cell-derived factor-1. J Biol Chem. 2001 Nov 23;276(47):43503-8. Epub 2001 Sep 24. PubMed PMID: 11571304.
Storage Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Range 62.5-4000 pg/ml
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