NBK1-19033 ND OLLAS / DYKDDDDK Epitope Tag Kit

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  • NBK1-19033

Product Description for ND OLLAS / DYKDDDDK Epitope Tag Kit

Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for ND OLLAS / DYKDDDDK Epitope Tag Kit

Product Category ELISA Kits
Quantity 2 vials
Synonyms ECS Epitope Tag, ECS tag, FLAG Epitope Tag, FLAG tag
Reactivity All Species
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Research Areas: Epitope Tags
Application Immunocytochemistry, immunofluorescence, Immunohistochemistry-Frozen, immunoprecipitation, Western Blot, This kit is useful for immunofluorescence, immunoprecipitation, Western blot, immunocytochemistry, and immunohistochemistry-frozen sections.
Background The pCMV-N-DYK-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.
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